细胞内微丝骨架的变化对人肝癌Bel-7402细胞形态的影响  

The Effect of Change in Cellular Microfilament Cytoskeleton on Cellular Morphology of Human Bel-7402 Hepatocarcinoma Cell

作　　者：林珏龙[1] 陈耀文[1] 李璟[2] 方昆阳[1] 许险峰[1] 霍霞[1] 

机构地区：[1]汕头大学医学院中心实验室,中国广东汕头515031 [2]汕头大学医学院生物化学与分子生物学教研室,中国广东汕头515031

出　　处：《激光生物学报》2004年第3期224-227,共4页Acta Laser Biology Sinica

摘　　要：癌细胞的形态及癌细胞的生物学行为(如粘附、迁移和浸润)与细胞内骨架系统密切相关,本研究利用Phalloidin FITC标记人肝癌Bel 7402细胞内F 肌动蛋白(F actin),使用激光扫描共聚焦显微镜观测细胞微丝骨架与癌细胞形态的关系,及Cyt B处理Bel 7402细胞后,癌细胞内微丝骨架的变化。结果显示:癌细胞内F actin解聚,F actin小体的聚集重组是癌细胞的形态变化的主要因素之一。Cellular morphology and cellular biological activities (i,e,. adhesion, mobility and invasion) are related to intracellular cytoskeleton. In the present study we used Phalloidin-FITC to label cellular F-actin of human Bel-7402 hepatocarcinoma cell. Intracellular microfilament of Bel-7402 cell and the change of cancer cellular microfilament cytoskeleton after Bel-7402 cell being treated with Cyt-B was observed by confocal scanning laser microscope (CSLM). The results showed that the morphological change of cancer cell was due to F-actin being depolymerized and actin protein being recombined to form the F-actin bodies.

关 键 词：激光扫描共聚焦显微镜 人肝癌Bel-742细胞 F-肌动蛋白 微丝骨架 细胞松弛素-B 

分 类 号：Q631[生物学—生物物理学] Q245