活性氧自由基对心肌细胞损伤效应研究  

The Cardiomyocytes Injury Efficiency Induced by Hydrogen Peroxide

作　　者：林灼锋[1] 李校坤[1] 孟娟[2] 

机构地区：[1]暨南大学药学院生物制药教研室,广州510632 [2]暨南大学医药生物研究开发中心,广州510632

出　　处：《中国生物工程杂志》2004年第6期81-85,共5页China Biotechnology

基　　金：国家"8 63"计划项目( 2 0 0 1AA2 15 13 1);国家"十五"创新药物重大专项基金资助项目

摘　　要：为探讨活性氧自由基对心肌细胞的影响 ,采用胰蛋白酶酶消化法分离SD乳鼠心肌细胞 ,培养于适当的条件并观察其形态学和生理学方面的特征 ;加入H2 O2 活性氧刺激心肌细胞 ,模拟氧自由基损伤心肌细胞方式 ,构建心肌细胞缺血再灌注损伤的模型并了解H2 O2 对心肌细胞的损伤作用。结果表明胰蛋白酶消化分离的心肌细胞能够在体外完好生长 ,并能够在一段时间内维持其原有的生理特性 ;MTT检测结果和形态学观察结果表明H2 O2 对心肌细胞的损伤与其浓度和作用时间呈正比关系 ,TUNEL和DNA凝胶电泳分析结果显示 ,H2 O2 在心肌细胞中的积累是造成细胞凋亡的主要因素之一。To study the cardiomyocytes injury efficiency induced by hydrogen peroxide, isolated the SD neonatal rat cardiac myocytes by trypsin enzyme digestion method, and cultured in suitable medium, observed the physiological character of cardiomyocytes, add some different concentration hydrogen peroxide to induce them, the change of cellar morphology were observed by reverse microscopy, viability were measure by MTT method and the rat of cardiomyocytes apoptosis were detected terminal deoxynucliotidyl transferase mediated dUTP nick end labeling (TUNEL) and DNA agarose gel electrophoresis analysis. The results indicated that there is a positive relation between the cardiomyocytes injury and the concentration of H 2O 2 the same as the stimulation times by H 2O 2; H 2O 2 is one of important factor to the cardiomyocytes apoptosis.

关 键 词：心肌细胞培养 H202 氧自由基 心肌细胞损伤 

分 类 号：R331.31[医药卫生—人体生理学]