L-赖氨酸高产菌株的选育及发酵培养基优化  

Screening for a high-yield of L-lysine producing mutant E_(0.9～37)and studying under fermentative conditions

作　　者：齐秀兰[1] 曾绍钧[1] 周忠喜[2] 

机构地区：[1]沈阳药科大学制药工程学院,辽宁沈阳110016 [2]沈阳药科大学高等职业学院,辽宁沈阳110026

出　　处：《沈阳药科大学学报》2004年第4期311-313,320,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的以L 赖氨酸产生菌钝齿棒杆菌 (Corynebacteriumcrenatum)D60 -95为出发菌株 ,提高L 赖氨酸产量。方法采用紫外线诱变处理 ,对大量抗七叶苷 (esculin)突变株进行初筛、复筛 ;在此基础上 ,利用均匀设计方法对发酵培养基成分进行优化组合。结果得到抗性突变株E0 9-3 7,其发酵液中L 赖氨酸产量较出发菌株提高 2 0 6 % ;发酵培养基主要成分最佳配比为葡萄糖 14 0g·L-1,硫酸铵 5 5 g·L-1,碳酸钙 5 0 g·L-1和盐酸豆饼水解液 18g·L-1。结论以上方法是选育L 赖氨酸高产菌株的有效途径。Objective To enhance the yield of L-lysine using L-lysine producing Corynebacterium crenatum D 60-95 as the initial strain.Method Ultraviolet mutation was employed to screen resistant mutants to esculin; on the basis,using the uniform design to optimize the ferment medium.Result The high-yield stable strain E 0.9-37 was obtained and the yield of lysine in the fermentation broth was increased by 20.6%; the optimal medium consisted of glycose 140 g·L -1,ammonium sulfate 55 g·L -1,calcium carbonate 50 g·L -1,hydrochloric acid-soybean cack hydrolyzate 18 g·L -1.Conclusion The method may serve as a valid approach to screen the strain with the high-yield of L-lysine.

关 键 词：钝齿棒杆菌 诱变育种 七叶苷 均匀设计 

分 类 号：R933[医药卫生—生药学]