二氧化碳存在下甲烷氧化细菌催化甲烷生物合成甲醇  

Biosynthesis of Methanol from Methane by Methanotrophic Bacteria in the Presence of Carbon Dioxide

作　　者：崔俊儒[1] 辛嘉英[1] 牛建中[1] 夏春谷[1] 李树本[1] 

机构地区：[1]中国科学院兰州化学物理研究所羰基合成和选择氧化国家重点实验室,甘肃兰州730000

出　　处：《催化学报》2004年第6期471-474,共4页

基　　金：国家自然科学基金重点项目 ( 2 993 3 0 40 );国家重点基础研究发展规划项目 (G19990 2 2 40 6)

摘　　要：在甲烷单加氧酶和脱氢酶系的作用下 ,甲烷氧化细菌MethylosinustrichosporiumIMV 30 11可以把甲烷氧化成二氧化碳 .在反应体系中充入一定比例的二氧化碳后 ,检测到了甲醇的积累 .混合气中CO2 ,CH4,O2 和N2 的体积比为 2∶1∶1∶1时甲醇的积累量达到最大 .在超滤膜反应器中进行了连续反应 ,利用反应混合气产生的压力将生成的甲醇从反应体系中分离 .连续反应Methanotrophs can oxidize methane to carbon dioxide through sequential reactions catalyzed by a series of enzymes including methane monooxygenase, methanol dehydrogenase, formaldehyde dehydrogenase, and formate dehydrogenase. When a suspension of methanotrophic bacteria of Methylosinus trichosporium IMV 3011 was incubated at 32 ℃ with methane and oxygen, there was an extracellular accumulation of methanol from methane oxidation in response to the carbon dioxide addition. The maximal methanol accumulation was achieved when 40% carbon dioxide was in the mixed reaction gas. Continuous reaction was performed in an ultrafiltration membrane reactor and the optimum mixed gas containing 20% methane, 20% oxygen, 20% nitrogen and 40% carbon dioxide was used as substrates and to maintain the transmembrane pressure. The methanol product was removed by the eluate buffer. The initial methanol concentration in the eluate was 8 22 μmol/L. The bioreactor was allowed to operate continuously for 198 h without obvious loss of productivity.

关 键 词：甲烷氧化细菌 甲基弯菌 生物催化 甲烷 生物合成 甲醇 二氧化碳 超滤膜反应器 

分 类 号：O643[理学—物理化学]