氯吡格雷对实验性动脉粥样硬化形成的影响  

Effects of Clopidogrel on the Formation of Experimental Atherosclerosis

作　　者：顾晴[1] 陈纪林[1] 阮英茆[2] 司文学[2] 王清峙[2] 徐新林[2] 褚雁[2] 张连庄[2] 陈在嘉[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院冠心病诊治中心,北京市100037 [2]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院病理科,北京市100037

出　　处：《中国循环杂志》2004年第2期145-148,共4页Chinese Circulation Journal

摘　　要：目的 :观察抗血小板药物氯吡格雷对实验性动脉粥样硬化形成的影响。方法 :2 9只雄性日本大耳白兔分为正常组 (n =9)、模型组 (n =10 )和氯吡格雷组 [4mg/(kg·d) ,n =10 ]。后两组建立高胆固醇饲料并免疫损伤诱发的主动脉粥样硬化模型。测血脂和血清C 反应蛋白 (CRP)浓度。观察主动脉组织病理形态学改变并定量分析病变程度 ,免疫组化法测定斑块部位的巨噬细胞和平滑肌细胞阳性细胞百分数。结果 :与模型组相比 ,氯吡格雷组的主动脉斑块面积 /内膜面积减少 2 7 5 2 % ,内膜厚度和内膜厚度 /中膜厚度分别减少 5 4 5 2 %和 5 0 98% ,斑块中巨噬细胞百分数从 ( 5 7 5 0± 8 40 ) %减至 ( 4 0 93± 7 0 8) % ,平滑肌细胞百分数从( 3 7 2 8± 3 3 5 ) %增至 ( 5 3 16± 6 96) % ,血清CRP浓度从 ( 2 67 86± 49 89)ng/ml降至 ( 189 82± 77 0 2 )ng/ml,均有显著性差异 (P <0 0 5～ 0 0 1)。结论 :氯吡格雷能够减轻动脉炎症反应 ,抑制动脉粥样硬化的形成。Objective: To observe the effects of clopidogrel on the formation of experimental atherosclerosis. Methods: Twenty-nine male rabbits were divided into three groups: normal group ( n =9),model group( n =10) and clopidogrel group 4 mg/(kg·d)( n =10). Rabbits of last two groups were fed on 2% cholesterol diet for 12 weeks and were injected with 10% Albumin Bovine. Serum lipids and C-reactive protein (CRP) were detected. The aortas were harvested for histomorphometric observation and quantitative analysis. The positive percentage of smooth muscle cells and macrophage cells was detected by immunohistochemistry. Results: Compared with model group,clopidogrel group showed that the ratio of lesion to intimal area decreased by 27.52%,intimal thickening and intima-to-media ratio respectively reduced by 54.52% and 50.98%,the positive percentage of macrophage cells decreased from 57.50±8.40% to 40.93±7.08%,the positive percentage of smooth muscle cells increased from 37.28±3.35% to 53.16±6.96%,and concentration of serum CRP decreased from 267.86±49.89 ng/ml to 189.82±77.02 ng/ml( p <0.05—0.01). Conclusion: Clopidogrel can suppress vascular inflammation and inhibit the formation of atherosclerosis.

关 键 词：氯吡格雷 实验性动脉粥样硬化 炎症 血脂 C-反应蛋白 

分 类 号：R543.5[医药卫生—心血管疾病]