乙型肝炎病毒DNA转染人T淋巴细胞株的研究  

TRANSFECTION OF CULTIVE LYMPHOCYTE LINE WITH CLONED CIRCULAR HEPATITIS B VIRUS DNA

作　　者：郭建平[1] 王茜[1] 王宇[1] 冯百芳[1] 陶其敏[1] 

机构地区：[1]北京医科大学人民医院肝病研究所

出　　处：《北京医科大学学报》1993年第1期21-23,共3页Journal of Peking University(Health Sciences)

摘　　要：本文试用磷酸钙DNA共沉淀法将HBV DNA转染于培养的人T淋巴细胞株-CEM细胞中,HBV可在转染后的细胞中复制和转录,转染后的细胞培养液中,用酶联免疫吸附法可检测出HBsAg,细胞中可检测出3.2Kb和2.2Kb的HBV DNA片段,HBV DNA对体外培养的人 T淋巴细胞株的成功转染将有助于HBV致病机理的研究。HBV DNA has been transfected the in vitro human lymphocyte line-CEM cells, using coprecipita-tes of calcium phosphate and DNA. After transfection, HBV can replicated and transcripted in the tr-ansfected cells. HBsAg encoded by HBV DNA can be detected in the culture medium of transfectedCEM cells by enzyme linked immunosorbent assay. 3.2kb and 2.2 kb segments of HBV DNA can beshowed using the Southern blotting method.

关 键 词：乙型肝炎病毒 转染 细胞培养 

分 类 号：R373.21[医药卫生—病原生物学]