两种检测乙型肝炎病毒核酸的聚合酶链反应比较  

Comparing the methods of testing hepatitis B virus deoxyribonucleic acid with Common Polymerase Chain Reaction and Fluorescence Quantify Polymerase Chain Reaction

作　　者：李侃 费克香[1] 刘华[1] 何汉[2] 

机构地区：[1]长江大学医学院医学实验中心,湖北荆州434000 [2]长江大学附属荆州市第一人民医院PCR室,湖北荆州434000

出　　处：《湖北省卫生职工医学院学报》2004年第1期79-80,共2页Journal of Hubei Medical Staff College

摘　　要：目的 :评价普通聚合酶链式反应 (C PCR)和荧光定量聚合酶链式反应 (FQ PCR)检测乙型肝炎病毒核酸 (HBVDNA)的临床应用价值。方法 :用C PCR和FQ PCR分别检测 2 4 9例乙型肝炎患者和 5 1例健康体检者血清HBVDVA。乙型肝炎血清学标志物用ELISA测定。结果 :用C PCR和FQ PCR检测HBVDVA阳性率分别为 :HBeAg (+)组 80 5 0 %和 10 0 0 0 % (P <0 0 1) ;抗HBe (+)组 17 39%和 82 6 0 % (P<0 0 1) ;HBeAg ( )抗HBe ( )组 14 5 1%和 4 0 32 % (P <0 0 1)。健康体检者两法均为阴性。结论 :FQ PCR对乙型肝炎诊断、传染性的判断和抗病毒药物疗效评价方面比C PCR更具有临床应用价值。Objective : To evaluate methods of testing hepatitis B virus deoxyribonucleic acid with Common Polymerase Chain Reaction and Fluorescence Quantify Polymerase Chain Reaction through clinical trial. Methods: Hepatitis B virus deoxyribonucleic acid were tested in 249 serum samples of hepatitis B virus patients and 51 normal peoples with Common Polymerase Chain Reaction and Fluorescence Quantify Polymerase Chain Reaction. Serological signs of hepatitis B virus were tested by ELISA. Results: Positive rate of testing hepatitis B virus deoxyribonucleic acid with Common Polymerase Chain Reaction and Fluorescence Quantify Polymerase Chain Reaction is: HBeAg(+) groups 80.50% and 100%(P<0.01); Anti-HBe(+) groups 17.39% and 82.60%(P<0.01); HBeAg(-) Anti-HBe(-) groups 14.51% and 40.32%(P<0.01). Both results of testing samples of normal peoples with two methods were negative. Conclusion: Fluorescence Quantify Polymerase Chain Reaction is superior to Common Polymerase Chain Reaction in clinical diagnose and judging infectivity and effect of Anti-virus medicine of hepatitis B virus.

关 键 词：普通聚合酶链式反应 荧光定量聚合酶链式反应 乙型肝炎病毒脱氧核糖核酸 

分 类 号：R512.3[医药卫生—内科学]