MMP-3/TIMP-1在大鼠肢体再灌注后关节软骨损伤的作用  

Effects of MMP-3/TIMP-1 in Rat Ischemia-reperfusion Articular Cartilage

作　　者：周建伟[1] 王成琪[2] 周继红[1] 张伟旭[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所四室,重庆市400042 [2]解放军第89医院全军创伤骨科研究所

出　　处：《骨与关节损伤杂志》2004年第5期325-327,共3页The Journal of Bone and Joint Injury

摘　　要：目的　探讨缺血再灌注后关节软骨中MMP - 3/TIMP - 1比例变化与软骨损伤的关系。方法　采用大鼠后肢股动脉夹闭的方法模拟缺血再灌注的动物模型 ,用Wistar大鼠 4 0只 ,随机分成正常对照组 (NG)、肢体单纯缺血组 (IG)和缺血再灌注组 (IR)。运用免疫组化技术 ,分别测定TIMP - 1和MMP - 3在关节软骨中不同时相的表达变化并进行半定量分析 ,观察关节软骨病理改变及蛋白多糖 (PG)的变化。结果　缺血再灌注后 ,关节软骨中的MMP - 3和TIMP - 1表达均有增加 ,但MMP - 3增加的幅度大于TIMP ,导致MMP - 3/TIMP - 1比值增大 ,与再灌注后引起的关节软骨损伤相关。Objective To investigate the relationship between the ratio of MMP-3 to TIMP-1 and articular cartilage injury after ischemia-reperfusion.Methods Rats hind limber femoral arteries were clapped and then loosen as an animal ischemia-reperfusion model.40 Wistar rats were divided into three groups:normal control group (NG),ischemia group(IG),ischemia-reperfusion group(IR).The expressions of TIMP-1 and MMP-3 were studied with immunohistochemistry and analyzed semiquantively at different time point after ischemia-reperfusion.The cartilage pathologic progresses and proteoglycans changes were observed.Results After ischemia-reperfusion,the expressions of MMP-3 and TIMP-1 both increased,but expression of MMP-3 increased more quickly than that of TIMP.Ratios of MMP-3 to TIMP-1 increased,showing an imbalanced expression related with articular cartilage injury.Conclusion The imbalance expression of TIMP/MMP-3 is an important factor in articular cartilage injury after ischemia-reperfusion injury.

关 键 词：MMP-3 TIMP-1 大鼠 肢体再灌注 关节软骨损伤 显微外科 

分 类 号：R681.3[医药卫生—骨科学] R-332[医药卫生—外科学]