低剂量苯并芘作用下传代培养人胚肺二倍体细胞的超微结构特征  

ULTRASTRUCTURE FEATURES OF DIPLOID CELL OF HUMAN EMBRYO LUNG IN VITRO UNDER LOW DOSE OF CARCINOGEN

作　　者：张枢贤[1] 杨晓林[1] 张桂芝[1] 唐素恩[2] 桑倩 张烨 闻仲权[3] 阎淑琴[3] 

机构地区：[1]北京医科大学公共卫生学院预防医学教研室 [2]北京医科大学第一临床医学院电镜室 [3]卫生部北京生物制品研究所

出　　处：《北京医科大学学报》1993年第5期329-331,395,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：探讨了体外连续培养的人胚肺二倍体细胞在低剂量苯并芘长时期作用下超微结构的早期癌特征。结果发现:低剂量(10^(-7)及10^(-8)mol)苯并芘(Bap)长时期(23～38代)作用下,光镜检查未见细胞有明显癌变征,而电镜下发现细胞核外形不规则,核膜深陷,有者呈复杂折叠及套叠,出现细桥伴分叶核形成,以及核内胞浆包涵体(或称核内假包涵体),核内的常染色质一般丰富;核仁巨大或多核仁等癌细胞特征。说明用电镜可观察到在致癌物作用下细胞早期癌变的征象。实验结果对进一步研究致癌物在低剂量、长时期作用下的致癌机理和预防措施将有重要意义。This paper approachs the early cancer characteristic of ultrastructure features of human embryolung under Benzo(a)pyrene(Bap) with low dose and long term. No obvious indication of carcinomacan be found by light microscope. However, by means of electron microscope, some early cancer char-icteristics can be observed as follows: irregular appearance of nucleus, complex folds and invagenationsof nuclear envelope, abundant euchromatin, giant nucleoli and nucleolus, the formation of tiny bridgesin company with nuclear lobulus and intranuclear pseudoinclusions as well. This experimental rcsultplays an important role in the further research of carcinogenic mechanism and prevention measurmensunder the action of carcinogen with low dose and long term

关 键 词：肺 二倍体细胞 培养 超微结构 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]