比较丹参酮ⅡA、ATRA和As_2O_3对NB_4细胞PML-RARα融合蛋白作用的规律  

Compare with the Influence of Tanshinone ⅡA、ATRA and As_2O_3 on the PML-RARα Protein in NB 4 Cells

作　　者：黄纯兰[1] 羊裔明[1] 刘志刚[1] 孟文彤[1] 刘霆[1] 张杰[1] 

机构地区：[1]四川大学华西医院血液科,四川成都,610041

出　　处：《华西医学》2004年第2期222-223,共2页West China Medical Journal

摘　　要：目的 :比较丹参酮ⅡA、ATRA和As2 O3 对NB4细胞PML -RARα融合蛋白作用的规律 ,探索TanⅡA降低PML -RARα融合蛋白水平的原因。方法 :药物作用 2 4、 72、 12 0h ,用间接免疫荧光方法检测PML -RARα融合蛋白的变化。结果TanⅡA影响NB4细胞PML -RARα荧光染色颗粒的规律与ATRA作用一致 ,与As2 O3 作用不一致。结论 :TanⅡA可能通过激活caspase裂解PML -RARα融合蛋白或通过PML -RARα融合蛋白中RARα的AF - 2部分而降解PML -RARα融合蛋白 ,致PML -RARα融合蛋白水平降低 ,NB4细胞分化。Objective:To explore the mechanism of the attenuation of PML-RAR α protein induced by Tan ⅡA,we compare with the influence of Tanshinone ⅡA?ATRA and As 2O 3 on the PML-RAR α protein in NB 4 cells.Method:The PML-RAR α protein in NB 4 cells induced by these drugs were measured by immuno-fluorescence antibodies against PML at the time of 24,72,120 hours.Result:Tan ⅡA and ATRA had the same regulation on the attenuation of PML-RAR α protein in NB 4 cells.But Tan ⅡA and As 2O 3 had different regulation on it.Conclusion:The mechanism of the attenuation of PML-RAR α protein probably is that Tan ⅡA degrades AF-2 part on RARα of PML-RAR α protein or that Tan ⅡA activates caspase to cleave PML-RARα protein.In the end,Tan ⅡA induces differentiation of NB 4 cells.

关 键 词：丹参酮ⅡA ATRA AS2O3 NB4细胞 PML-RARΑ融合蛋白 问接免疫荧光法 

分 类 号：R285[医药卫生—中药学]