内皮素、内皮素转化酶在自体静脉移植术后内膜增生中的作用  

Effect of endothelin-converting enzyme and endothelin-1 on intimal hyperplasia in venous autograft

作　　者：宋清斌[1] 段志泉[1] 辛世杰[1] 罗英伟[1] 张海强[1] 张健[1] 

机构地区：[1]中国医科大学附属第一医院血管外科,沈阳110001

出　　处：《中华普通外科杂志》2004年第5期283-285,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目 ( 3 0 0 0 0 163 )

摘　　要：目的探讨内皮素 1(endothelin 1,ET 1)及内源性内皮素转化酶 (endothelinconvertingenzyme ,ECE)在自体静脉移植术后内膜增生中的作用。 方法建立自体静脉移植模型 ,采用反转录聚合酶链反应 (RT PCR)和免疫组织化学方法 ,对ECE、ET 1和增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)在移植静脉的表达情况进行分析。 结果移植术后 6h ,吻合口处静脉中膜即出现PCNA阳性平滑肌细胞 ,并随着时间推移逐渐增高 ,在 1～ 2周左右达到高峰。 2周后 ,PCNA阳性SMC开始减少 ,于术后 8周趋于平稳。移植血管ET 1、PCNA主要在平滑肌细胞中表达。在mRNA水平 ,随着移植术后时间的推移 ,ECE的表达逐渐增高 ,术后 1～ 2周达到高峰 ,在 8周左右趋于平稳 ,ET 1与ECE密切相关 (r =0 975 )。结论ET 1、ECE的动态变化过程具有相关性 ,ECE可能通过ECE→ET 1→SMC通路促进内膜增生。Objective To investigate changes of endothelin-1(ET-1) and endothelin-converting enzyme (ECE) in different time intervals after autograft vein implantation. Methods A model of autogenous vein graft was established by interposition of the jugular vein into abdominal aorta in 80 Wistar rats. RT-PCR and immunohistochemistry were employed to test mRNA and protein level of ECE, ET-1 and proliferating cell nuclear antigen (PCNA). Results Positive PCNA appeared at 6 hours after transplantation, with time reaching a peak at 1 to 2 week. ECE mRNA increased with time reaching a peak after 1-2 weeks and stabilizing around 8 weeks. ET-1 expression underwent similar tendence with ECE, reaching a peak after 1-2 weeks and stabilizing at 8 weeks at the protein level. Expression of ET-1 and ECE were closely related by the time pattern after vein autograft (r=0.975). Conclusions The process of intimal hyperplasia in its occurrence and pattern of change are related with dynamics of ET-1 and ECE. ECE may lead to intimal hyperplasia of the autografted vein through a passway of ECE to ET-1 to SMC.

关 键 词：内皮素 内皮素转化酶 自体静脉移植 术后并发症 内膜增生 增殖细胞核抗原 

分 类 号：R654.3[医药卫生—外科学]