肾移植患者尿液中单核细胞趋化性肽-1浓度变化的意义  

Determination of urinary monocyte chemotactic peptide-1 in renal transplant recipients

作　　者：孙星慧[1] 谭建明[1] 吴卫真[1] 方玉华[2] 李黔生[2] 严伟[1] 徐廷昭[1] 周浩[1] 

机构地区：[1]南京军区福州总医院泌尿外科,福州350025 [2]第三军医大学附属大坪医院肾移植中心

出　　处：《中华器官移植杂志》2004年第3期153-154,共2页Chinese Journal of Organ Transplantation

摘　　要：目的　探讨肾移植受者尿液中单核细胞趋化性肽 1(MCP 1)浓度变化的意义。方法　用生物素 抗生物素蛋白复合物酶联免疫吸附试验 (ABC ELISA法 )检测 5 0例肾移植受者术后不同时间尿液中MCP 1的含量。结果　移植术后肾功能稳定者尿液中的MCP 1水平为 (416± 2 1) μg/L ,正常对照组为 (40 8± 11) μg/L ,二者间的差异无显著性 (P >0 .0 5 ) ;术后发生急性排斥反应者的MCP 1水平为 (1195± 5 8) μg/L ,明显高于正常对照组和肾功能稳定者 (P <0 .0 1) ;抗排斥治疗有效者的MCP 1水平在冲击治疗 1周后即明显下降 (P <0 .0 1) ,而无效者的MCP 1水平无变化。结论　检测尿液中的MCP 1水平有助于急性排斥反应的诊断及预后判断。Objective To evaluate monocyte chemotactic peptide-1 (MCP-1) excretion levels in urine of renal transplant recipients and study the relation between urinary MCP-1 levels and acute rejection.Methods Urinary MCP-1 levels were determined by avidin biotin complex-enzyme linked immunosorbent reaction (ABC-ELISA).Results The urinary MCP-1 levels were significantly higher in recipients with acute rejection than in clinically stable ones (P< 0.01) and normal subjects (P< 0.01). In those patients with acute rejection who responded to the treatment, urinary MCP-1 levels were reduced significantly after antirejection.Conclusion The urinary MCP-1 excretion correlates with acute renal rejection. An increase in urinary MCP-1 excretion may represent an early signal of ongoing acute renal rejection. And MCP-1 may be a useful marker for early diagnosis and responsiveness after antirejection treatment.

关 键 词：肾移植 尿液 单核细胞趋化性肽-1 移植物 趋化因子 排斥反应 

分 类 号：R699.2[医药卫生—泌尿科学]