霉酚酸酯对未成熟树突状细胞抗原递呈功能的影响  

Influences of MMF on the presentation functions of in vitro cultured dendritic cells

作　　者：李怀富[1] 张明[2] 许泽清[3] 韩从辉[1] 闵志廉[2] 郑克立[1] 

机构地区：[1]中山大学附属第五医院泌尿外科,珠海519000 [2]第二军医大学附属长征医院器官移植科 [3]第二军医大学附属长征医院核医学科

出　　处：《中华器官移植杂志》2004年第3期179-182,共4页Chinese Journal of Organ Transplantation

摘　　要：目的　研究霉酚酸酯 (MMF)在体外对小鼠未成熟树突状细胞抗原递呈功能的影响。方法　在骨髓来源未成熟树突状细胞的培养过程中加入MMF ,表型鉴定之后 ,做抗原递呈功能分析 ,观察抗原特异性增殖反应、混合淋巴细胞反应以及T淋巴细胞抗原特异性低反应性。结果　以MMF处理的未成熟树突状细胞向T淋巴细胞递呈可溶性抗原的能力减弱 ,在同基因型的树突状细胞和T淋巴细胞接触条件下不能刺激致敏T淋巴细胞的有效增殖 ;MMF处理的树突状细胞在体外的同种混合淋巴细胞培养反应中能够维持树突状细胞于未成熟状态 ,不能充分活化CD4 + T淋巴细胞和诱发TH1反应 ,诱导了T淋巴细胞对同种抗原的低反应性 ;MMF处理的树突状细胞诱导的T淋巴细胞低反应性具有同种抗原特异性。结论　在体外培养过程中给予MMF干预能够降低未成熟树突状细胞的抗原递呈能力 ,增强同种未成熟树突状细胞的免疫耐受诱导作用。Objective To explore the influence of MMF on the presentation functions of in vitro cultured dendritic cells.Methods Bone marrow-derived dendritic cell progenitors from BalB/c mice were treated with MMF or without. The antigen-presenting capacities, antigen specific proliferation reaction, mixed lymphocyte reaction and antigen-specific hyporesponsiveness of T-cells were analyzed to investigate the influences of MMF on the presentation functions of dendritic cells.Results Treatment with MMF reduced the soluble antigen presenting capacities of DCs to T cells. The proliferation of syngeneic T cells co-cultured with MMF-DC was significantly depressed, and displayed a comparatively low level of MLR-sti- mulatory activity. The levels of T helper cells derived cytokines were depressed, too.Conclusion MMF has a suppressive influences on the antigen-presenting functions of in vitro cultured dendritic cells and promotes the tolerance-induction effect of DCp.

关 键 词：霉酚酸酯 未成熟树突状细胞 MMF 抗原递呈功能 DC T淋巴细胞 

分 类 号：R392[医药卫生—免疫学]