趋化因子RANTES对人外周血单个核细胞的免疫活化作用  

Immune active effects of chemokine RANTES on human peripheral mononuclear cells

作　　者：顾晓 唐孝达[2] 顾沈阳 杨尚琪[2] 周佩军[3] 徐达[3] 王祥慧[3] 谭建明[2] 

机构地区：[1]扬州大学临床医学院泌尿外科,225001 [2]上海市第一人民医院器官移植中心 [3]上海第二医科大学附属瑞金医院泌尿外科

出　　处：《中华器官移植杂志》2004年第3期183-185,共3页Chinese Journal of Organ Transplantation

基　　金：上海市医学领先专业重点学科发展基金资助 ( 9830 0 5)

摘　　要：目的　探讨趋化因子RANTES刺激对人外周血单个核细胞 (PMNC)的免疫活化作用及其内在机制。方法　分离人外周血 ,应用不同终浓度的人重组RANTES(rhRANTES)以及抗CD3单克隆抗体 (抗CD3mAb)进行体外刺激 ,并对增殖反应显著者给予吡咯啉烷二甲基硫脲 (PDTC)或CTLA4Ig进行干预。采用3 H 胸腺嘧啶核苷掺入法检测PMNC增殖程度 ,流式细胞仪检测淋巴细胞表型的变化。结果　rhRANTES终浓度为 10 0ng/ml和 5 0 0 0ng/ml时 ,诱导PMNC增殖反应出现两次峰值 ;rhRANTES(10 0ng/ml)刺激产生的增殖反应显著高于抗CD3mAb(5 0ng/ml)的刺激 (P <0 .0 5 ) ,但两者无拮抗或协同作用 ;PDTC和CTLA4Ig对rhRANTES(10 0ng/ml)诱导的增殖反应均能产生抑制作用 ,并呈现剂量依赖性 ;rhRANTES刺激后 ,淋巴细胞CD2 5表达率显著增加 (P <0 .0 5 ) ,而人RANTES的受体CCR5表达率显著降低 (P <0 .0 5 ) ,CD2 8表达率及CD4 /CD8比值无明显改变(P >0 .0 5 )。结论　RANTES趋化信号具有不依赖于CD3信号的独特的诱导PMNC免疫活化的功能。Objective To investigate the proliferation and phenotypes of peripheral mononuclear cells (PMNC) stimulated by recombinant human RANTES (rhRANTES) and the mechanisms involved.Methods PMNC was stimulated by various concentrations of rhRANTES and/or anti-CD3 mAb and intervened by PDTC and CTLA4Ig. Scintillation counter was used to count the cpm of proliferation cells and flow cytometry was used to detect the phenotypes of lymphocytes.Results rhRANTES was capable of directly stimulating purified human PMNC proliferation and two peaks occurred with rhRANTES concentration of 100 ng/ml and 5000 ng/ml respectively. The proliferation of PMNC stimulated by rhRNATES was significantly higher than that in the presence of anti-CD3mAb (P< 0.05). The immune active effects of rhRANTES could be inhibited by PDTC or CTLA4Ig in a dose dependent manner. RANTES treatment of PMNC in vitro increased the level of cell surface CD25 and decreased the CCR5 expression. But it did not influence the ratio of CD4/CD8 and CD28 expression of lymphocytes.Conclusion RANTES is an important stimulatory mediator in human PMNC activation. This special signal works depending on the activation of IL-2 signal pathway, CD28 costimulatory pathway and nuclear factor-κB, but independent of CD3 activation.

关 键 词：趋化因子 RANTES 外周血 单个核细胞 免疫活化作用 PMNC 免疫活性 信号传递 

分 类 号：R392[医药卫生—免疫学]