银杏外种皮多糖对HL-60细胞的体外实验研究  

Experimental Study of Ginkgo biloba Exocarp Polysaccharides on HL-60 Cells in Vitro

作　　者：许爱华[1] 陈华圣[1] 孙步蟾[1] 

机构地区：[1]扬州大学医学院,扬州225001

出　　处：《中药材》2004年第5期361-363,共3页Journal of Chinese Medicinal Materials

基　　金：江苏省社会发展基金(No BS2000086);江苏省教育厅自然科学基金(No 99KJD360001)

摘　　要：目的:研究银杏外种皮多糖对体外HL-60细胞的作用。方法:运用MTT方法和流式细胞技术,检测HL-60细胞体外增殖、凋亡以及相关基因的表达。结果:银杏外种皮多糖(40～160mg/L,48h)可抑制HL-60细胞增殖及增殖促进基因c-myc的表达,可诱导HL-60细胞凋亡并下调凋亡抑制基因bcl-2的表达。结论:银杏外种皮多糖对HL-60细胞增殖及凋亡的影响涉及增殖促进基因c-myc和凋亡抑制基因bcl-2。Objective:To study the effect of Ginkgo biloba exocarp polysaccharides(GBEP) on HL-60 cells in vitro. Method:Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of HL-60 cells. Result:GBEP(40～160mg/L,48h) could inhibit HL-60 cells proliferation and the expression of proliferation-promotion gene cmyc, induce HL-60 cells apoptosis and down-regulate the expression of apoptosis-inhibitory gene bcl-2. Conclusion: Influence of GBEP on HL-60 cells proliferation and apoptosis may relate to its effects on the expression of proliferation-promotion gene c-myc and apoptosis-inhibitory gene bcl-2.

关 键 词：银杏外种皮多糖 HL—60细胞 体外实验 中药 

分 类 号：R285.5[医药卫生—中药学]