轮状病毒DNA疫苗经不同途径接种诱导体内免疫应答研究  

Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine

作　　者：张静[1] 王宁遂[2] 邓兵[2] 朱静[2] 

机构地区：[1]重庆医科大学儿科学院心内科 [2]重庆医科大学儿科研究所,重庆400014

出　　处：《重庆医科大学学报》2004年第3期308-310,共3页Journal of Chongqing Medical University

摘　　要：目的 :通过研究不同途径接种轮状病毒DNA疫苗所诱导的体内免疫应答 ,寻找其适合的免疫途径。方法 :轮状病毒基因疫苗 pcDNA1/VP7经肌肉注射及鼻粘膜两种途径免疫BALB/c小鼠 ,利用ELISA方法对其诱导产生的体液免疫应答进行测定。结果 :经肌肉注射及鼻粘膜两种途径接种轮状病毒DNA疫苗后 ,均在BALB/c小鼠体内诱导产生了病毒特异性IgG增高 (P <0 .0 5 ) ,而病毒特异性IgA与对照组相比无显著性差异。结论Objective:To search for the efficient immune routes for rotavirus DNA vaccine by evaluating efficacy in inducing immune responses to rotavirus.Methods:In this study BALB/c mice were immunized by intramuscular and intranasal routes with rotavirus DNA vaccine,pcDNA 1/VP7,then rotavirus specific systemic immunity was measured.Results:Serum rotavirus specific IgG but not IgA titers of mice immunized by both routes were significantly higher than that of the unimmunized controls.Conclusion:Both intramuscular and intranasal routes are candidates for rotavirus DNA vaccine.

关 键 词：轮状病毒 DNA疫苗 粘膜免疫 

分 类 号：R373[医药卫生—病原生物学] R364.7[医药卫生—基础医学]