生长激素对大鼠心肌缺血/再灌注后心肌细胞凋亡及凋亡相关基因NF-κB蛋白表达的影响  

Apoptosis and related gene expression of NF-κB protein in rat with experimental myocardial ischemia/reperfusion injury

作　　者：黄瑞健[1] 孙培吾[1] 林乌拉[1] 吴伟康[1] 

机构地区：[1]中山大学第一附属医院心外科

出　　处：《中国急救医学》2004年第6期420-422,共3页Chinese Journal of Critical Care Medicine

基　　金：福建省自然科学基金资助项目部分内容 (NO C0 0 10 0 10 )

摘　　要：目的　研究生长激素 (growthhormone ,GH)对大鼠心肌缺血 /再灌注 (IR)后心肌细胞凋亡及相关基因NF -κB蛋白表达的影响。方法　 2 4只大鼠随机分为 3组 ,假手术组 (n =8,仅手术 2 4h)、心肌缺血 /再灌注 (IR)组 (n =8)、GH组 (n =8) ,后两组均缺血 30min ,再灌注 2 4h ,其中GH组的每只大鼠实验术前皮下肌注GH 1U/kg体重 ,连续 7d ,其中第 7次皮下注射于手术前进行。前两组每只大鼠相应皮下肌注生理盐水 0 .5mL/d。检测心肌细胞凋亡及心肌细胞核NF -κB蛋白表达并进行心肌组织病理学检查。结果　大鼠心肌IR 2 4h后心肌细胞凋亡指数明显上升 (对照于假手术组 ,P <0 .0 5 ) ,心肌细胞核NF -κB蛋白表达呈阳性染色指数明显升高 (P <0 .0 5 ) ,心肌病理检查见心肌缺血区呈大小不一的坏死孔灶 ,缺血心肌间有炎症细胞浸润 ,心肌排列不整齐 (HE染色 ) ,而GH组心肌细胞凋亡率及心肌细胞核NF -κB蛋白表达呈阳性染色指数明显好于IR组 (P <0 .0 5 ) ,心肌细胞炎症细胞也明显减少 ,坏死灶也少于IR组。结论　GH可以减少心肌缺血 /再灌注后心肌细胞凋亡及细胞核NF -κB染色阳性指数 。Objective To study effect of growth hormone on myocardial apoptosis and related gene expression of NF-κB protein in rat with experimental myocardial ischemia/reperfusion injury.Methods Twenty-four rats were divided randomly into three groups , sham operation group (n=8, only operation for 24 h), IR group (n=8) and GH group (n=8) , 30 minutes's ischemia followed by 24 hours' reperfusion were performed in IR group and GH group . The rats in GH group were treated with subcutaneous injection of GH 0.1 U/kg body weight per day for 7 days and the seventh injection began at pre-operation, and the rats in other groups were treated with subcutaneous injection of normal saline 0.5 mL per day individually. Terminal deoxynucleotidyl transferase mediated dUTP fluorescein nick and tabeling (TUNEL) and DAB immunohistochemical staining were used to detect the changes of apoptosis and protein expression of NF-κB respectively. Histopathological changes in myocardium was observed by HE dye . Results Apoptotic index (AI) and positive dyed index (PDI) of NF-κB protein increased obviously in IR group than that of sham operation group (P<0.05). Local myocyte necrosis and inflammatory cell infiltration in IR group were also observed . On the contrary, AI and PDI of NF-κB protein in GH group were better than that of IR group ( P <0.05) . Local myocyte necrosis and inflammatory cell infiltration was diminished more obviously in the GH group than that of IR group. Conclusion The AI and PDI of NF-κB protein in myocardial cell nuclear lessened obviously due to usage of GH which indicated GH can protect myocardial after IR.

关 键 词：缺血 再灌注 生长激素 NF-ΚB 心肌细胞 细胞凋亡基因 

分 类 号：R541[医药卫生—心血管疾病]