异烟肼比色法测定麻地喷雾剂中地塞米松磷酸钠含量  

Determination of dexamethasone sodium phosphate in ephedrine and dexamethasone nasal cavity spray by isoniazid colorimetry

作　　者：方焱[1] 胡世林[1] 柳涌[1] 

机构地区：[1]安徽省立医院药剂科,合肥230001

出　　处：《安徽医药》2004年第1期44-46,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的　建立异烟肼比色法测定麻地喷雾剂中地塞米松磷酸钠含量的方法。方法　根据地塞米松磷酸钠具有△1 ,4 3 酮甾体结构 ,与异烟肼发生缩合反应显黄色 ,在 4 0 4nm处有紫外吸收峰的特点 ,测定其含量。结果　地塞米松磷酸钠在 77 8～1 1 6 7mg·L-1 范围内 ,吸收度与浓度呈良好的线性关系 (r =0 .9998,n =5 )。平均回收率 1 0 0 .4 % ,RSD为 0 6 4 %。日内RSD =1 35 % ,日间RSD =1 88% (n =5 )。结论　方法简便易行、快速准确 。AIM To establish a method for determination of dexamethasone sodium phosphate in nasal cavity spray by isoniazid colorimetry. METHODS The content of dexamethasone sodium phosphate can be determined by isoniazid colorimetry based on the △ 1,4 3 ketosteroid in skeleon could be condensated with isoniazid , which implied absorption move to 404 nm. RESULTS The linear range of dexamethasone sodium phosphate was 77 8～116 7 mg·L -1 with a correlation coefficient of 0.9 998 . The average recovery was 100 4% with RSD of 0 64% .The intra day RSD was 1 35% and the inter day RSD was 1 88%. CONCLUSION The method is fast and accurate and can be used for quality control of pharmacy.

关 键 词：异烟肼比色法 测定 麻地喷雾剂 地塞米松磷酸钠 含量 

分 类 号：R927.2[医药卫生—药学]