喜树茎尖组织培养与植株再生  

Tissue culture of shoot-tip and plantlet regeneration of Camptotheca acumunata

作　　者：吕立堂[1] 朱冬雪[1] 赵德刚[1] 

机构地区：[1]贵州大学农业生物工程省级重点实验室,贵州贵阳550025

出　　处：《中草药》2004年第6期682-684,共3页Chinese Traditional and Herbal Drugs

基　　金：贵州省科学技术基金资助 ( 2 0 0 2 3 0 3 3 ) ;贵州省科技厅专项基金 (黔科合字 ( 2 0 0 3 ) JN0 12 )

摘　　要：目的　探索喜树的人工快繁。方法　以喜树幼苗的茎尖作为外植体 ,培养在附加不同激素的培养基上。结果　以 B5培养基附加 6 - BA 0 .2 m g/ L ,IBA 0 .0 5 mg/ L和 AS (afenine sulfate,10 mg/ L )对丛生芽的诱导与增值效果最佳 ,而附加 IBA0 .5 mg/ L,KT0 .1mg/ L 和 AS10 m g/ L 的生根效果最佳。试管苗移栽到珍珠岩 -土壤(3∶ 7)的基质中生长良好 ,成活率高达 96 %。结论　为喜树的开发利用提供了一条新途径。Object To explore the artificial propagation of the medicinal plant, Camptotheca acuminata Decne. Methods The shoot-tip was tested as the explants and cultured on culture media with different portions of hormone. Results The best medium for bud induction was the B5 basic medium with additions of 6-BA (0.2 mg/L), IBA (0.05 mg/L), and AS (afenine sulfate, 10 mg/L). While B5 with the additions of IBA (0.5 mg/L), KT (0.1 mg/L), and AS (10 mg/L) was suitable for rooting. The seedling was cultivated and grew well on the base material mixed with perlite-soil (3∶7). The survival rate of transplant was up to 96%. Conclusion The above mentioned method provides a new effective way to exploit this plant resources.

关 键 词：喜树 植株再生 组织培养 

分 类 号：R282.13[医药卫生—中药学]