丽江山慈菇RAPD遗传变异初探  

Preliminary analysis on genetic variation of Iphigenia indica by RAPD

作　　者：王海英[1] 虞泓[1] 李南高[2] 何显静[1] 张金渝[1] 周利杰[1] 

机构地区：[1]云南大学生命科学院生态遗传学实验室,云南昆明650091 [2]云南英茂生物技术实验室,云南昆明650106

出　　处：《中草药》2004年第6期685-688,共4页Chinese Traditional and Herbal Drugs

基　　金：昆明制药集团资助项目 ;云南大学省级生物技术人才培养基地资助项目

摘　　要：目的　研究丽江山慈菇 Iphigenia indica遗传变异及遗传结构。方法　采用 RAPD技术 ,对产自云南的 3个居群进行 DNA指纹检测。结果　筛选出 2 0个随机引物 ,对 3居群扩增共产生 12 0条 DNA片段 ,在 0 .2～ 3kb,其中 71条谱带具有遗传多态性 ,约占 5 9.17% ,平均每个引物扩增的 DNA带数是 3.5 5。 Shannon index在丽江玉龙雪山居群 (Ca)为 0 .1994 ,大理宾川鸡足山居群 (Cb)为 0 .2 0 0 7,丽江永胜片角镇居群 (Cc)为 0 .2 5 4 8;居群平均水平为 0 .2 183,物种水平为 0 .2 886。 Nei's genetic identity在 Ca和 Cb居群间为 0 .930 9;在 Ca和 Cc居群间为 0 .932 7;在 Cb和 Cc居群间为 0 .94 6 6。居群间基因分化系数 Gst为 0 .2 0 4 4。结论　 RAPD分析可为丽江山慈菇保护对策和措施的制定 ,遗传育种和 GAP种植方法及标准的制定 。Object To study on the genetic diversity and genetic structure of Iphigenia indica Kunth. Methods Random amplified polymorphic DNA (RAPD) was applied to detect DNA fingerprints of three populations of I. indicafrom Yunnan Province. Results Twenty primers were screened, and a total of 120 DNA fragments were amplified ranging from 0.2-3 kb, among which 71 (59.17%) were polymorphic. The average number of DNA band produced by each primer was 3.55. The Shannon index was (0.199 4) in Ca population, 0.200 7 in Cb population, 0.254 8 in Cc population, respectively; the average value of populations was 0.218 3. The Shannon index was 0.288 6 in species. Nei's genetic identity was 0.930 9 between Ca and Cb, 0.932 7 between Ca and Cc, 0.946 6 between Cb and Cc. G_(st) within population was (0.204 4.) Conclusion For the establishment of protective tactic and measure, and of the standard of good agriculture practice (GAP) growth and heredity breeding, RAPD analysis provides I. indicawith theoretical foundation and basal data.

关 键 词：丽江山慈菇 RAPD 遗传多态性 

分 类 号：R282.710.3[医药卫生—中药学]