肾病综合征出血热的成龄鼠模型  

ADULT MOUSE MODEL OF HEMORRHAGIC FEVER WITH RENAL SYNDROME

作　　者：姚楚铮[1] 黄莉莉[1] 张满新 刁保卫[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所,北京102206

出　　处：《病毒学报》1993年第2期152-155,共4页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：4～8周龄的近交系C57或杂交昆明系小鼠,脑内接种肾病综合征出血热(HFRS)病毒。在第-l、+1,+2、+4天注射环磷酰胺50mg/kg体重(第0天感染病毒),可使小鼠由隐性感染变为急性致死性感染,病死率为94％。在病死动物的脑,肺、肝、脾和肾等器官中,检查翻HFRS病毒抗原。这些器官显示微血管系统和实质组织的病变。这种成龄鼠模型可用于本病的研究。Intracerebral infection of hemorrhagic fever with renai syndrome(HF-RS ) virus proves highly pathogenic for newborn mice, but harmless for adult mice. We observed that cyclophosphamide ( CY )-immunosuppressed adult mice were Susceptible to acute infection leading to death.4-8 week old C57 mice or outbred Kunming strain mice inoculated in-tracerebrally with HFRS virus A-16 strain and immunosuppressed with four 50mg/kg CY doses at days -1, +1, + 2, + 4 (day O:virai infection), developed a lethal disease(94% mortality ) , and were mori-bund by 14-18 days postinfection. Specific virai antigen was regularly found in brain, lung, spleen, liver and kidney by indirect IF assay. His-topathologic study showed vascular changes including hyperemia, hemorr-hage, exudate, degeneratiem and necrosis of parenchymal tissues of brain, lung, liver and kidney. Based on these findings, We suggest that C57 mice or kunming strain mice may be used as an animal modei for inves-tigation of the disease.

关 键 词：肾病综合征 出血热病毒 动物模型 

分 类 号：R373.32[医药卫生—病原生物学]