用水貂阿留申病毒单克隆抗体对该病毒蛋白抗原性的分析  

ANALYSIS OF ANTIGENICITY OF MINK ALEUT IAN DISEASE VIRUS ( ADV ) PROTEINS USING MONOCLONAL ANTIBODIE S

作　　者：吴威[1] 聂金珍[1] Bloom M.E. 

机构地区：[1]中国农业科学院特产研究所,吉林市132109 [2]美国NIH落基山研究室

出　　处：《病毒学报》1993年第3期236-240,共5页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：制备的5株抗阿留申病毒(Aleutian disease virus,ADV)G株单克隆抗体(Y9、Y19、Y13、Y21和B4),都具有中和能力,都能和经乙醚处理的ADV-G发生中和反应。通过Y9与ADV-G7个基因克隆表达的抗原反应,确定了一个中和抗原决定簇编码核酸位于2750-3439碱基之间,并确定对流免疫电泳(CIEP)抗原结合蛋白位点在32k和25k蛋白上。以上工作为制备亚单位疫苗提供了理论基础。McAb Y9, Yl9, Yl3, Y21 and b4 against ADV-G can neutralize ADV-G treated with other. Reaction of McAb Y9 with the products of seven DNA clones of ADV-G confirmed that the gene fragment coding an ADV-G neutralization epitope is located between the 2750-3439 ge nomic sequence. We found that the combining protein epitopes of CIEP antigen is located in the 32k and 25k prote頽s. This provided theory tical basis for a subunit vaccine.

关 键 词：水貂 阿留申病毒 单克隆抗体 蛋白 

分 类 号：S852.65[农业科学—基础兽医学]