在我国流行的脊髓灰质炎中发现脊灰病毒Ⅰ型自然重组株  

DISCOVERY OF A RECOMBINANT OF POLIOVIRUS TYPE 1 FROM PARALYTIC POLIOMYELITIS CASES IN CHINA

作　　者：方肇寅[1] O.Kew 任斌[1] 郑渡平[2] 温乐英[1] 杨辰夫 苏锦[1] 陈美光[3] 郑焕英[4] 孟宪坤 张振国 刘复林 H.Yoshikura 

机构地区：[1]中国预防医科院病毒学研究所,北京100052 [2]美国疾病控制中心 [3]河南省卫生防疫站 [4]广东省卫生防疫站 [5]内蒙古自治区卫生防疫站 [6]河北省卫生防疫站 [7]大同矿务局防疫站 [8]日本国立预防卫生研究所

出　　处：《病毒学报》1993年第3期195-202,共8页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：脊髓灰质炎病毒(PV)是引起脊髓灰质炎的最主要的病毒。我们在研究PV1野毒株的分子流行病学时,发现了一株PV1自然重组病毒。用Sabin 1特异PCR检定该毒株基因组2505～2601区段,或用两个Sabin 1特异RNA探针分别打点杂交检定641～740和2502～2575区段时,推断它可能为野毒株;而测定VP1/2A连接区段3296～3445序列时,又表明它为疫苗株。向5′端扩大测序,从3262起的5′侧有与我国PV1野毒株相同的许多核苷酸取代,证明它为野毒株和疫苗株的重组病毒,重组位点为3262/3263。实验中建立了检测该重组病毒的特异的PCR方法。检测我国10个省市、自治区47份PV1分离株中,5个省区14份麻痹病例由重组病毒感染引起。这些重组株在我们测序的3155～3445区段序列彼此几乎相同(同源性≥96.7％),推断它们可能是某病儿受野毒株、疫苗株双重感染产生重组病毒后的子代病毒。有关该重组病毒的毒力、免疫原性、传播性等正在进一步研究中。Polivirus ( PV ) is the main etiological a靍nt of paralytic poliomye-litis. A recombinant of PV1 was identified during the molecular epide-miological survey by using sequence analysis of the genomic PV1/2A junc tion region ( residues 3296-3445) . The PVl isolates were tested at the beginning by Sabin 1-specific PCR and Dot hybridization and was ex-cluded from vaccine-related PV1. Its sequencing data of the 150 nucleotides shared 97% homology to Sabin 1. Expanded sequencing of its 5'-side re vcaled many nucleotide substitutions identical to those of wild PV1 from residue3262 upward,which indicates the recombination site at resi-dues 3262/3263 junction. 14 of 57 PV1 isolates collected from 13 provinces or autonomous regions were found to be recombinant by a recombi-nant-specific PCR developed by us. The high sequence similarity among them suggests that these 14 PV1 isolates probably were the progeny of a recombinant produced by a patient dually infected by wild and Sabin 1. Studies of virulence, immunogenicity, transmissibility etc. of this recombinant are going on.

关 键 词：脊髓灰质炎病毒 重组病毒 

分 类 号：R373.22[医药卫生—病原生物学]