软骨藻酸对H4细胞氨基酸释放和Ca^(2+)浓度的影响  

Effects of domoic acid on amino acid release and intracellular free Ca^(2+) in H4 Cell

作　　者：王斌[1] 李龙[1] 石年[1] 陈亮[1] 

机构地区：[1]华中科技大学同济医学院卫生毒理学系,湖北武汉430030

出　　处：《卫生毒理学杂志》2004年第2期77-79,共3页Journal of Health Toxicology

基　　金：湖北省自然科学基金资助 (2 0 0 2AB1 2 3)

摘　　要：目的　研究软骨藻酸 (domoicacid)对H4细胞的兴奋性、抑制性氨基酸释放和胞内游离Ca2 + ( [Ca2 + ]i)浓度的影响。方法　应用高效液相色谱 (HPLC)和荧光分光光度计检测 0、0 0 64、0 64和 6 4μmol/L软骨藻酸作用于细胞 2h后的兴奋性、抑制性氨基酸释放和 [Ca2 + ]i浓度。结果　天冬氨酸和谷氨酸 :中、高剂量组均高于对照组 ,差异有显著性(P <0 0 5 ) ;甘氨酸 :仅高剂量组高于对照组 ,差异有显著性 (P <0 0 5 ) ;γ 氨基丁酸 :低、中和高剂量组均低于对照组 ,差异有显著性 (P <0 0 5 ) ;兴奋性氨基酸 /抑制性氨基酸 :低、中和高剂量组均高于对照组 ,差异有显著性 (P <0 0 5 )。胞内[Ca2 + ]i :低、中和高剂量组分别为 ( 2 0 8 65± 11 0 1)、( 3 42 3 1± 15 0 8)和 ( 5 81 3 6± 17 2 4)nmol/L ,高于对照组 [( 14 3 2 5±11 97)nmo1/L] ,差异有显著性 (P <0 0 1)。兴奋性氨基酸与 [Ca2 + ]i浓度有显著的正相关性 (r =0 93 2 0P <0 0 1)。结论　软骨藻酸能引起H4细胞兴奋性和抑制性氨基酸释放和胞内 [Ca2 + ]i变化 。Objective To study the effect of domoic acid on amino acid release and intracellular free Ca 2+([Ca 2+]i) in H4 cell.Methods HPLC and spetroflurometer were applied to measure the contents of excitatory amino acid(EAA), inhibitory amino acid (IAA),and [Ca 2+]i in H4 cell respectively exposured to 0, 0.064, 0.640, 6.40 μmol/L domoic acid after 2h.Results Asparagic acid and Glutaminic acid: The contents of medium-dose group and high-dose group were significantly different from that of control group(P<0.05);Glycine: The content of high-dose group was significantly different from that of control group(P<0.05); Gamma-amino butyric acid: The contents of all dose groups were significantly different from that of control group (P<0.05). EAA/IAA: The values of all dose groups were significantly higher than that of control group (P<0.05) . [Ca 2+]i: The values of low-dose group, medium-dose group, and high-dose group were respectively(208.65±11.01),(342.31±15.08) and (581.36±17.24)nmol/L,increased significantly compared with (143.25±11.97)nmol/L of control group (P<0.01).There were significant positive correlations(r= 0.932 0, P<0.01)between the contents of EAA and that of [Ca 2+]i.Conclusion Domoic acid can cause the changes of extracellular EAA and IAA contents and intracellar [Ca 2+]i in H4 cell cultured in vitro,which may be the important mechanism of excitatory toxicity for domoic acid.

关 键 词：软骨藻酸 H4细胞 氨基酸释放 CA^2+浓度 神经胶质细胞 

分 类 号：R992[医药卫生—毒理学]