镍离子跨人红细胞膜转运的动力学及其机制  

Kinetics and mechanisms of transference across membrane of human erythrocytes by nickel ions

作　　者：乔秀文[1] 吴刚[2] 李荣昌[3] 王夔[3] 

机构地区：[1]石河子大学师范学院化学系,新疆石河子832002 [2]内蒙古科技大学医学部化学教研室 [3]北京大学药学院化学生物学系

出　　处：《卫生毒理学杂志》2004年第2期89-91,共3页Journal of Health Toxicology

基　　金：国家自然科学基金资助项目 (2 9571 0 0 7

摘　　要：目的　研究镍离子 (Ni2 + )跨人红细胞膜转运动力学和转运机制 ,以及Ni2 + 对红细胞膜的作用。方法　利用转运动力学方法测定Ni的跨人红细胞胞膜转运速率 ,改变Ni2 + 的浓度、溶液pH值、转运促进剂和特异抑制剂 ,研究这些因素对红细胞摄入Ni2 + 的影响 ,并利用荧光动力学扫描方法观察Ni2 + 对膜蛋白内源性荧光的影响。结果　人红细胞摄入Ni2 + 的一级动力学常数kNi2 + =( 2 0× 10 - 3) h ,Ni2 + 对细胞膜蛋白的内源性荧光影响很小。结论　Ni2 + 是以简单扩散方式跨细胞膜转运 。Objective To study the kinetics and mechanisms of transference across the membrane of human erythrocytes by nickel ions (Ni 2+) and effect of Ni 2+ with the membrane of human erythrocytes.Methods The rate of transmembrane transport of Ni 2+ was determined by the method of kinetics, and the influence on uptake of nickel by human erythrocytes had been studied by the variety of the concentration of Ni 2+ and pH of solution system and the transport promoter and inhibitor.The influence of Ni 2+ on membrane of erythrocytes was studied by kinetics of fluorescence scan.Results The first-order kinetic rate constant was k Ni 2+=(2.0×10 -3)/h.The effect of Ni 2+ on intrinsic fluorescence of membrane proteins was small.Conclusion Ni 2+ passes through the membrane by passive diffusion and the it's effect on membrane of erythrocytes is weakly.

关 键 词：镍离子 人红细胞膜 动力学动力芋 跨膜转运 

分 类 号：R331.141[医药卫生—人体生理学]