中国人丁型肝炎病毒抗原编码区基因的cDNA克隆和序列分析  

MOLECULAR CLONING AND SEQUENCING OF A HEPATITIS DELTA ANTIGEN-CODING cDNA FROM CHINA

作　　者：刘善虑[1] 易炎杰[1] 丛旭[1] 詹美云[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052

出　　处：《病毒学报》1993年第1期15-22,共8页Chinese Journal of Virology

基　　金：国家863高科技生物技术领域资助

摘　　要：从我国四川一丁型肝炎病毒抗原(HDAg)、HDV RNA双阳性的HBsAg携带者血清中提取RNA,采用人工合成的引物进行4次逆转录和聚合酶链反应(PCR),获得了长度分别为707bp、414bp、876bp和274bp的4个HDV cDNA片段,并对其进行了克隆和序列分析。其抗原编码区核苷酸序列与美国(Makino)、意大利(Wang)、英国(Saldanha)和我国台湾株(Chao)等株比较同源性,分别为99.4％、92.1％、94.3％和91.4％,由它推导的氨基酸序列的同源性分别为99.1％、87.9％、88.2％和89.3％,并发现了4个集中保守的区域。Chinese hepatitis delta antigen-coding cDNA was characterized by cloning and sequencing four fragments with size of 707bp, 414bp, 376bp and 274bp respectively, from a HBsAg carrier positive for HDAg and HDV RNA from Western China Sichuan Province, using reverse trans-cription and polymerase chain reaction. Comparison of homology of the Chinese HDAg-coding sequence with those of other known isolates: America ( Makino ) , Italia ( Wang ) , United Kindom ( Saldanha ) , Taiw-an(Chao)is 99.4%, 92.1%, 94.3%, 91.4% for nucleotide and 99.1%, 87.9%, 88.2%, 89.3% for amino acid respectively, and four conserved re靑ons were located.

关 键 词：丁型肝炎病毒 逆转录 聚合酶链反应 

分 类 号：R373.21[医药卫生—病原生物学]