高粱抗蚜虫SSR体系的建立  

The optimization of SSR technique system on greenbug resistance in sorghum

作　　者：夏雪岩[1] 常金华[1] 罗耀武[1] 李荣改[1] 刘国庆[1] 

机构地区：[1]河北农业大学农学院,河北保定071001

出　　处：《河北农业大学学报》2004年第3期21-24,共4页Journal of Hebei Agricultural University

基　　金：国家自然科学基金资助项目(30170498)

摘　　要：以抗蚜高粱———河农16为试材,采用单因素筛选的方法,摸索SSR反应体系中各个影响因素的浓度和用量,然后采用完全随机试验,进行优化组合,最终获得适宜高粱抗蚜虫基因定位的SSR体系。其中TaqDNA聚合酶0 3μL(5u μL),10×PCRBuffer(Mg2+)2 0μL,引物1 5μL(10μmol L),模板DNA60ng。利用该体系进行扩增,所得谱带清晰、稳定、非特异性带少。A high resistant material to greenbug in sorghum—Henong 16 was used in this expirement. Single factor selection was made to investigate better concentration in SSR technique system. Then, random thoroughly experiment was made to seek the best combination among factors. At last, the best SSR technique system was established. Taq DNA polymerase 0.3 μL(5 u/μL ),10×PCR Buffer(Mg^(2+)) 2.0 μL,primer 1.5 μL (10 μmol/L),template DNA 60 ng. Using this system, amplification bands were clear, standard and non-especial bands were shorter.

关 键 词：高粱 抗蚜 SSR 体系优化 

分 类 号：S514[农业科学—作物学]