松材线虫rNDA-ITS1区分子检测与鉴定  

Molecular identification of Bursaphelenchus xylophilus by PCR amplification of first internal transcribed spacer of ribosomal DNA

作　　者：李一农[1] 余道坚[1] 李芳荣[1] 谢伟宏[1] 陈杰[1] 张青汉[1] 

机构地区：[1]深圳出入境检验检疫局,深圳518000

出　　处：《植物保护》2004年第3期61-63,共3页Plant Protection

摘　　要：利用两对松材线虫特异性引物,分别对来自日本、美国和葡萄牙的松材线虫虫样进行PCR检测,成功扩增出330 bp和220 bp rDNA-ITS1区基因片段,该方法对松材线虫成虫或幼虫均能作出准确鉴定。DNA fragments of about 220bp and 330bp were amplified by PCR using two pairs of specific primers that were designed from the ribosomal DNA (rNDA). The genomic DNA of Bursaphelenchus xylophilus and Bursaphelenchus mucronatus originated from Japan, United States and Portugal was used as PCR template. This technique can be used to identify the adults or larval of Bursaphelenchus xylophilus exactly.

关 键 词：松材线虫 rNDA-ITS1 分子检测 PCR检测 鉴定 

分 类 号：S763.18[农业科学—森林保护学]