离子色谱脉冲安培检测法测定水体颗粒物与河口沉积物中的糖  

Determination of sugar from sediments and particles by ion chromatography-pulsed ampere detector.

作　　者：张培敏[1] 朱岩[1] 凌艳艳[1] 

机构地区：[1]浙江大学化学系,浙江杭州310028

出　　处：《浙江大学学报（理学版）》2004年第4期431-434,共4页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(20375035);浙江省自然科学基金资助项目(201089);浙江省分析测试基金资助项目(02164);国家海洋局海底科学开放实验室资助.

摘　　要：采用离子交换色谱-脉冲安培检测法,可在不需要衍生的条件下测定糖.本研究采用NaOH为淋洗液,Ion-PacAG4A-SC预柱,CarboPacTMPA10分离柱,金电极为工作电极,Ag/AgCl电极为参比电极,离子交换色谱脉冲安培检测法测定沉积物及颗粒物中的含糖量.各种糖分离效果较好,各组分的线性、重现性良好.该方法不仅具有较好的灵敏度和检测限,而且对痕量复杂组分样品分析,干扰少,测定效果好.Sugar can be directly determined without derivation reaction by ion exchange chromatography-pulsed ampere detector, in which the eluent is 22.5 mmol/L NaOH, a gold electrode is work electrode and Ag/AgCl electrode is reference electrode. L-rhamnose, L-arabinose, D-galactose, glucose, xylose ,D-fructose can be well separated. Experimental results show that the linear relationship and repeatability are good. The method is used to determine low component and complicated samples, and the results are satisfactory.

关 键 词：离子交换色谱 脉冲安培检测 单糖 沉积物 颗粒物 

分 类 号：O657.75[理学—分析化学]