葡聚六糖诱导后黄瓜幼苗细胞内Ca^(2+)水平变化的细胞化学研究  

Calcium Level Changes in Cells of Cucumber Seedlings Induced with Glucohexaose

作　　者：范海延[1] 陈伟芝[1] 李天来[1] 李宝聚[2] 

机构地区：[1]沈阳农业大学,辽宁沈阳110161 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《沈阳农业大学学报》2004年第2期127-129,共3页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(39870454);辽宁省科技攻关项目(2002215004);沈阳市重大项目(2001215001)

摘　　要：采用改进的焦锑酸钙细胞化学方法 ,探讨了葡聚六糖诱导后Ca2 + 在黄瓜幼苗细胞中超微结构定位变化动态。结果表明 ,在正常情况下 ,黄瓜幼苗的细胞壁和细胞间隙存在大量Ca2 + 。葡聚六糖诱导后2h,细胞壁和细胞间隙Ca2 + 沉淀物明显减少 ;诱导后4h ,细胞壁和细胞间隙只偶尔能看到有少量Ca2 + 沉淀物 ,细胞质中Ca2 + 沉淀物增多 ;诱导后7h ,Ca2 +The changes of Ca 2+ location in cells of cucumber seedlings after induced with glucohexaose were examined with the method of the improved calcium antimonate precipitation.The results showed that there was a large amount of calcium in cell wall and intercellular spaces under normal conditions.When the seedlings were induced with glucohexaose after2hrs,the calcium level in cell wall and intercellular spaces diminished.After4hrs,the deposits in cell wall and intercellular spaces intercellular spaces further diminished.Meanwhile the calcium level in the cytoplasm increased.After7hrs,the calcium returned to cell wall and intercellular spaces.

关 键 词：黄瓜 幼苗 细胞 CA^2+ 葡聚六糖 信使系统 

分 类 号：S642.201[农业科学—蔬菜学] Q942.6[农业科学—园艺学]