栓皮栎茎段离体培养的研究  

In vitro cultivation of nodal segments of the cork tree (Quercus variabilis)

作　　者：张存旭[1] 宋敏[2] 赵忠[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]曲阜师范大学生命科学院,山东曲阜273165

出　　处：《西北植物学报》2004年第7期1260-1265,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：西北农林科技大学科研专项基金(2001);陕西省科技攻关项目(2001K01-G7-01)

摘　　要：以4～6个月的实生苗茎段为外植体,研究了离体条件下培养基及激素对栓皮栎器官发生和植株再生的影响。结果表明:初代培养选用低盐培养基WPM、BTM和GD,附加0.2mg·L-16-BA,丛生芽较多,茎粗壮,生长势健壮,均好于高盐培养基MS。继代培养时,以BTM为基本培养基,添加0.2～0.6mg·L-16-BA有利于茎芽增殖和生长,当6-BA浓度为0.8～1.0mg·L-1时,不利于茎芽伸长。以WPM为基本培养基,附加NAA0.1mg·L-1和IBA0.25mg·L-1时,生根率高,根系发育好。Seedlings of 4～6 months were used as a source of initial explants.The effects of media and plant growth regulators on organogenesis and plantlet regeneration of Quercus variabilis in vitro were studied.The low salt media GD,WPM,BTM containing 0.2 mg·L^(-1) 6-BA are more suitable for initial culture than MS media richer in salts,in which it produced more shoots,thick stem and robust vigor.BTM medium supplemented with 0.2～0.6 mg·L^(-1) 6-BA in subculture was in favor of the shoot proliferation and growth.While 6-BA concentration in medium reached 0.8～1.0 mg·L^(-1),shoots elongation was prevented.High rooting percentages and excellent development root systems were obtained on WPM medium with NAA 0.1 mg·L^(-1) and IBA 0.25 mg·L^(-1) combination.

关 键 词：离体培养 茎段 植株再生 栓皮栎 

分 类 号：S511[农业科学—作物学]