Bt转基因玉米的获得及其对玉米螟的抗性分析  

Production of Bt transgenic maize resistant to corn borer

作　　者：张红伟[1] 张红梅[2] 郑祖平[1] 陈刚[1] 王国英[3] 谭振波[1] 

机构地区：[1]北京市农林科学院北京农业生物技术中心,北京100089 [2]山西省农业科学院玉米研究所,山西忻州034000 [3]中国农业大学生物技术国家重点实验室,北京100094

出　　处：《西北植物学报》2004年第7期1266-1270,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30270832);北京市高技术室基金项目(953850100)资助

摘　　要：以玉米幼胚和愈伤组织为受体,利用基因枪法将Bt基因GFMCry1A导入玉米杂交种"京玉7号(501/京24)"的亲本自交系501中,从转基因高世代材料中筛选出高抗玉米螟的转基因株系T123。T123及其与京24配制的杂交种的田间食叶级别均小于2级,而且也没有观察到玉米螟在茎杆中运动的隧道,表现出高抗玉米螟。The immature embryos and callus of elite inbred 501,one of the parents of commercial hybrid 'Jingyu 7 (501/Jing 24)',were bombarded with the plasmid carrying GFMCry1A by microprojectile bombardment.Transformants were obtained from resistant callus on selection medium containing 10 mg/L Bialaphos for 9 weeks.Transgenic line T123 was selected from the advanced progeny of transgenic plants carrying Bt gene.The evaluation result of leaf-feeding damage degree and stalk tunneling indicated T123 and its hybrid of T123/Jing 24 exhibited more resistant to corn borer than the non-transgenic controls in field.

关 键 词：优良自交系 基因枪 GFMCry1A 

分 类 号：Q943.2[生物学—植物学] S513[农业科学—作物学]