中华鳖非特异性免疫功能的群体差异研究  

A STUDY ON THE VARIATION OF NON-SPECIFIC IMMUNE FUNCTIONS AMONG POPULATIONS OF SOFT-SHELLED TURTLE TRIONYX SINENSIS

作　　者：刘至治[1] 蔡完其[1] 付立霞[1] 黄玲璘[1] 

机构地区：[1]农业部水产种质资源与养殖生态重点开放实验室上海水产大学,上海200090

出　　处：《水生生物学报》2004年第4期349-355,共7页Acta Hydrobiologica Sinica

基　　金：上海市水产办"中华鳖养殖业持续发展研究"项目 (科 0 0 95 )资助

摘　　要：从补体总量、红细胞C3 b受体花环率、红细胞天然免疫肿瘤细胞花环率、T淋巴细胞活性E花环率及白细胞吞噬活性五方面 ,探讨了黄河鳖、淮河鳖、洞庭湖鳖、鄱阳湖鳖及太湖鳖在非特异性免疫功能上的差异。主要结果 :(1)在补体总量及红细胞天然免疫肿瘤细胞花环率上 ,五群体之间差异不显著 (F <0 .0 5 ) ;(2 )在红细胞C3 b受体花环率上 ,黄河鳖显著地高于鄱阳湖鳖与太湖鳖 (P <0 .0 5 ) ,而其余群体间则差异不显著 (F <0 .0 5 ) ,花环率大小顺序为 :黄河鳖 >淮河鳖 >洞庭湖鳖 >鄱阳湖鳖 >太湖鳖 ;(3)在T淋巴细胞活性E花环率上 ,黄河鳖显著高于鄱阳湖鳖 (P <0 .0 5 ) ,太湖鳖极显著地低于其他四个群体 (F >0 .0 1) ,其余群体间则差异不显著 (P >0 .0 5 ) ,花环率大小顺序为 :黄河鳖 >淮河鳖 >洞庭湖鳖 >鄱阳湖鳖 >太湖鳖 ;(4 )在白细胞吞噬活性上 ,除淮河鳖与鄱阳湖鳖差异不显著外 (P >0 .0 5 ) ,其余群体间则差异极显著 (F >0 .0 1) ,活性大小顺序为 :黄河鳖 >洞庭湖鳖 >淮河鳖 >鄱阳湖鳖 >太湖鳖。综上所述 ,在非特异性免疫功能上 ,黄河鳖最强 ,太湖鳖较弱。The non-specific immune functions were studied among five populations of soft-shelled turtle from Yellow River,Huai River,Dongting Lake,Poyang Lake and Taihu Lake from five aspects--1) the total amount of complement, 2) the percentage of erythrocyte C 3b receptor rosette, 3) the percentage of erythrocyte native immune adhering tumor cell rosette, 4)the percentage of erythrocyte active rosette(EaR)of T lymphocyte and 5)the activity of leukocyte phagocyte The major results were:(1) There were no significant difference on the total amount of complement and the percentage of erythrocyte native immune adhering tumor cell rosette among the five populations of soft-shelled turtle (F<0.05).(2) On the percentage of erythrocyte C 3b receptor rosette,Yellow River population was significantly higher than that of Poyang Lake population and Taihu Lake population(P<0.05),and there was no significant difference among other populations(F<0.05).The order of rosette percentage was Yellow River population>Huai River population>Dongting Lake population>Poyang Lake population>Taihu Lake population.(3) On the percentage of EaR of T lymphocyte, Yellow River population was significantly higher than that of Poyang Lake population(P<0.05),Taihu Lake population was highly significantly lower than that of the other four populations(F>0.01),there was no significant difference among other populations(P>0.05).The order of EaR percentage was Yellow River population>Huai River population>Dongting Lake popuation>Poyang lake population>Taihu Lake population.(4) On the activity of leukocyte phagocyte,there was no significant difference between Huai River population and Poyang Lake population(P>0.05),while there was highly significant defference among other populations(F>0.01).The order of activity was Yellow River population>Dongting Lake population>Huai River population>Poyang Lake population>Taihu Lake population.From above all,it could be concluded that Yellow River population was the highest one on non-specific immune functions while Taihu L

关 键 词：中华鳖 非特异性免疫 群体差异 补体 红细胞C3b受体花环率 

分 类 号：S947.1[农业科学—水产养殖] Q959.63[农业科学—水产科学]