荧光测钙装置中单色激发光源的设计和应用  

Design and application of a monochromator for microfluorometric [Ca^(2+)]i measurement unit

作　　者：周云燕[1] 兰李[1] 杨志勇[1] 瞿安连[1] 徐涛[1] 

机构地区：[1]华中科技大学生命科学与技术学院生物物理与化学研究所,湖北武汉430074

出　　处：《光电工程》2004年第7期24-27,共4页Opto-Electronic Engineering

基　　金：国家自然科学基金资助项目(30327001 60071030);国家自然科学基金重点项目(30130230)

摘　　要：为了满足胞内荧光测钙实验的需要,设计了一种新型的单色激发光源。其中,短弧氙灯发出的光经椭球反射镜聚焦后采用抛物面反射镜准直成平行光,通过光栅的衍射分光后又经过会聚物镜把光能量会聚到光纤端面,光纤的另一端面输出单色光。采用光路偏折的方法,有效地克服了灯源的噪声。结果显示,波长为340nm和380nm的光能量分别可达到30mW和120mW,能很好地满足荧光测钙实验的要求。In order to meet the needs of microfluorometric measuring of intracellular [Ca2+]i , a new monochromator is designed. Wherein, the light from short-arc xenon lamp is first focused by an ellipsoidal mirror and then is collimated into parallel light through a paraboloidal mirror. The parallel beam is diffracted by plane grating and then converged at one end face of optical fiber by a convergent triple-lens, and monochromatic light is exported from another end face of fiber. The application of deflection optical path effectively eliminates noise of light source. The results show that the power of the light source with 340nm and 380nm wavelength attains 30micron and 120micron, respectively. They can fulfill the demands of microfluorometric [Ca2+]i measurement.

关 键 词：单色光源 光学设计 光学系统 荧光测钙 

分 类 号：TH74[机械工程—光学工程]