第1、2代酶联免疫试剂检测重型病毒性肝炎患者抗-HCV结果的比较  

The difference between the positive rate of anti-HCV in the patients with severe viral hepatitis detected by the first generation or second generation enzyme linked-immunosorbent assay

作　　者：张大志[1] 赵有蓉[1] 张全海[1] 王志毅[1] 秦波[1] 何华[1] 周智[1] 郭树华[1] 张定凤[1] 

机构地区：[1]重庆医科大学病毒性肝炎研究所,400010

出　　处：《中华肝脏病杂志》2004年第5期299-300,共2页Chinese Journal of Hepatology

摘　　要：目的 比较第1代和第2代酶联免疫(ELISA)检测试剂盒检测重型肝炎患者的抗-HCV阳性率。 方法 1984～1990年期间住院的重型肝炎患者78例,治疗前留置血清使用美国Ortho公司的第1代酶联免疫检测试剂盒检测抗-HCV;1997～2003年期间住院的重型肝炎患者251例,于治疗前使用国产第2代酶联免疫检测试剂盒(厦门新创)检测抗—HCV。 结果 使用第1代ELISA检测的抗-HCV阳性率为51.9％,使用第2代ELISA试剂检测的抗-HCV阳性率为1.2％,两组比较,差异有显著性(x^2=133.68,P≤0.001)。 结论 使用第2代ELISA试剂检测重型肝炎患者血清抗-HCV的阳性率明显低于第1代ELISA检测的阳性率。Objective To compare the positive rate of antibody to hepatitis C virus(anti-HCV) in sera of patients with severe viral hepatitis between 1984～1990 year and 1997-2003 year. Methods Serum anti-HCV was detected by enzyme linked-immunosorbent assay(ELISA). It was detected by the first generation(1st) ELISA (Ortho Co. USA) in 79 cases of severe viral hepatitis during 1984～1990 year, and it was detected by the second generation(2nd) ELISA(Xiamen Xingchuang Co. China)in 251 cases of severe viral hepatitis during 1997-2003 year. Results The positive rate of serum anti-HCV was 51.9% detected by the 1st ELISA in 79 cases of severe viral hepatitis during 1984～1990 year, and it was 1.2% detected by the 2nd ELISA in 251 cases of severe hepatitis during 1997～2003 year(x^2=133.68, P≤0.001). Conclusion To compare with the positive rate of serum antibody to hepatitis C virus in severe viral hepatitis detected by the 1st ELISA, it was lower that detected by the 2nd ELISA.

关 键 词：丙型肝炎病毒 抗-HCV阳性率 酶联免疫吸附试验 发病机制 HCV抗体免疫印迹 核酸放大试验 

分 类 号：R373.21[医药卫生—病原生物学] R512.6[医药卫生—基础医学]