大鼠中枢和外周神经损伤后胞体分布区神经组织的红外光谱分析  

Infrared spectroscopic analysis of the neuronal somas in adult rats after central and peripheral nerves injury

作　　者：刘淑红[1] 吕艺[2] 吴海涛[1] 宋占军[3] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]解放军第304医院创伤研究室,北京100037 [3]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《军事医学科学院院刊》2004年第2期143-145,148,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目 ( 3 9970 2 4)

摘　　要：目的 :观察外周和中枢神经损伤后胞体分布区神经组织总蛋白和核酸的变化规律。方法 :应用傅里叶红外光谱 (FTIR)检测方法 ,分析大鼠脊髓和坐骨神经损伤后相应的神经元胞体分布区神经组织中归属为核酸和蛋白质的谱带吸收强度的变化。结果 :伤后早期 ,外周和中枢神经损伤后其相应胞体部位神经组织RNA ,DNA和蛋白质的含量均增加 ;1周后 ,外周和中枢神经元胞体分布区RNA ,DNA和蛋白质含量的变化规律不同 :外周神经元胞体RNA和DNA的含量仍较高 ,而中枢神经伤侧和对照侧胞体RNA和DNA的含量接近 ,中枢和外周神经元胞体蛋白含量变化相反。结论 :外周和中枢神经损伤后再生过程中其胞体反应性不同 。Objective: To observe the changing pattern of total protein and nucleic acid contents in the neuronal somas after central and peripheral nerves injury in rats. Methods: The change of protein and nucleic acid contents in corresponding CNS and PNS neuronal somas was analyzed by means of Fourier transform infrared spectroscope after the spinal cord and sciatic nerve injury in rats. Results: At the early stage of injury, the contents of proteins and nucleic acids in both CNS and PNS neuronal somas were increased. One week later, nucleic acid contents in injured PNS neuronal somas remained high, while there was no difference between the control and injured sides in CNS neuronal somas. The change of protein content in CNS and PNS was on the contrary. Conclusion: The responses of neuronal somas in PNS and CNS were different following injury, which might be associated with the difference in regeneration between PNS and CNS injury.

关 键 词：创伤 神经系统 傅里叶变换红外光谱学 脊髓损伤 坐骨神经 

分 类 号：R651.2[医药卫生—外科学]