氯乙烯染毒大鼠DNA损伤与肝脏某些生化指标的变化  

DNA damages and changes of certain hepatic biochemical indexes in rats exposed to vinyl chloride monomer

作　　者：朱守民[1] 王爱红[1] 刘桂明[1] 孙祖越[1] 金复生[2] 周元陵[2] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院,上海20003 [2]复旦大学附属金山医院职防科,上海200032

出　　处：《卫生研究》2004年第3期273-275,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .30 0 70 650 ) ;科技部973项目 (No .2 0 0 2CB51 2 90 9)

摘　　要：目的　研究氯乙烯 (VCM)对大鼠肝细胞和血淋巴细胞DNA的损伤作用 ,及肝脏某些生化指标的变化。方法　DNA损伤采用单细胞凝胶电泳 (彗星实验 )法 ,代谢酶活性采用分光光度法 ,肝功能测定使用自动生化分析仪。结果　彗星发生率染毒组显著高于对照组 ,肝细胞和血淋巴细胞彗星发生率显著相关。结论　VCM可导致大鼠肝细胞和血淋巴细胞DNA发生损伤 ,且存在剂量 效应 反应关系。Objective To investigate DNA damages of liver cells and lymphocytes of rats exposed to vinyl chloride monomer(VCM), to examine the changes of hepatic function, and certain biochemical indexes. Methods DNA damages were detected by single cell gel electrophoresis (comet assay). The activities of metabolizing enzymes were measured by spectrophotography, and hepatic function was determined by biochemical analyser. Results The incidence rates of comet cells in the experimental groups were significantly higher than those of the controls; and the rates of comet cells between liver cells and lymphocytes among the groups were closely correlated. Conclusion VCM could induce DNA damage of liver cells and lymphocytes, where was a dose-effect/response relationship.

关 键 词：氯乙烯 DNA损伤 彗星实验 

分 类 号：Q593.1[生物学—生物化学] Q592.1