共轭亚油酸对肥胖大鼠PPARγ基因表达及血清瘦素水平的影响  

Effect of conjugated linoleic acid on PPARγgene expression and serum leptin in obese rat

作　　者：周晓蓉[1] 孙长颢[1] 姜丽英[1] 王海英[1] 刘荣[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150001

出　　处：《卫生研究》2004年第3期307-309,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .30 2 71 1 1 8)

摘　　要：目的　研究不同剂量共轭亚油酸 (CLA)对饮食诱导肥胖大鼠PPARγ基因、瘦素、血糖、血脂的影响。方法　选用雄性Wistar大鼠 ,随机分为对照组、高脂组、高脂 +CLA组 (每 10 0g饲料含CLA分别为 0 75g、1 5 0g、3 0 0g) ,于第 12周末处死动物 ,计算脂 体比 ,测定大鼠血糖、血脂及瘦素水平 ,并应用RT PCR的方法检测大鼠白色脂肪组织过氧化物酶体增殖物激活受体γ(PPARγ)的表达水平。结果　CLA可降低肥胖大鼠血糖、甘油三酯 (TG)、总胆固醇 (TC)及瘦素水平 ,增加脂肪组织PPARγmRNA的表达水平。结论　CLA可降低肥胖大鼠血糖、血脂 ,并可通过激活PPARγ下调瘦素水平 ,有改善肥胖大鼠的瘦素抵抗作用。Objective In order to study the effect of conjugated linoleic acid on PPARγgene expression, serum leptin,blood glucose and blood lipid level.Methods Five groups of male Wistar rats were randomly selected and fed with different diets (basic diet, high-fat diet, high-fat with 0.75%CLA, high-fat with 1.50%CLA, and high-fat with 3.00%CLA) for 12 weeks. At the end of experiment, the rats were sacrificed,the ratio of lipids to body weight were calculated,and the blood glucose, serum lipids, serum leptin were measured. RT-PCR was used for measuring the expression of PPARγmRNA in white adipose tissue of rats.Results It was shown that the supplement of CLA could decrease decreased blood glucose, serum triglyderide, cholesterol and leptin levels, and increased the expression of PPARγ in white adipose tissue of obese rat.Conclusion CLA could decrease blood glucose, serum lipids, and decrease the levels of leptin possibly via activating peroxisome proliferator -activated receptor-γ(PPARγ), resulting in the improvement of leptin resistance of obese rat.

关 键 词：瘦素 过氧化物酶体增殖物激活受体Γ 血糖 血脂 

分 类 号：R589.2[医药卫生—内分泌] Q493.9[医药卫生—内科学]