蛋白质组研究技术平台的建立——双向电泳条件的建立及优化  

Establishment of two-dimensional gel electrophoresis technical platform for proteome research

作　　者：刘建军[1] 房师松[1] 李习艺[1] 陈晓燕[1] 李喜梅[1] 赵锦[1] 庄志雄[1] 

机构地区：[1]深圳市疾病预防控制中心,深圳518020

出　　处：《卫生研究》2004年第3期327-330,共4页Journal of Hygiene Research

基　　金：广东省自然科学基金资助项目 (No .34970 ) ;国家"973"计划资助项目 (No .2 0 0 2CB51 2 90 4 )

摘　　要：目的　建立和优化蛋白质组研究的双向电泳及相关技术。方法　以固相pH梯度等电聚焦为第一向和垂直SDS PAGE为第二向 ,对样品制备、上样量、IPG胶条、等电聚焦和聚丙烯酰胺凝胶电泳程序及参数、染色方法等相关技术进行了比较研究和条件优化。结果及结论　成功地得到了人胚肺成纤维细胞和蚕卵蛋白质组双向电泳图谱 ,建立了蛋白质组研究的双向电泳技术平台。Objective To establish and optimize two-dimensional gel electrophoresis technique for proteomics. Methods Immobiline pH gradients isoelectric focusing was used as 1D,vertical SDS-PAGE as 2D,some factors such as method and volume of loading sample, choice of gels, concentration of SDS gels, preset of electric program and parameters, protocol for staining the gels were improved. Results and Conclusion Using the proper method described above, satisfactory 2DE maps of human embryonic lung fibroblast(HLF)and silkworm egg were obtained. 2DE technical platform for proteome research was set up.

关 键 词：蛋白质组 技术平台 双向凝胶电泳 

分 类 号：Q51[生物学—生物化学] Q-3