氢化物发生-原子荧光法测定细胞内痕量锑  

Determination of trace antimong in cells by hydride generation-atomic fluorescence spectrometry

作　　者：解清[1] 王京宇[1] 王晓燕[1] 王耐芬[1] Christian Brochu Marc Ouellette 

机构地区：[1]北京大学公共卫生学院,北京100083 [2]加拿大拉瓦尔大学传染病研究中心

出　　处：《卫生研究》2004年第3期347-349,共3页Journal of Hygiene Research

基　　金：北京大学公共卫生学院中心仪器室与加拿大拉瓦尔大学传染病研究中心合作项目

摘　　要：为了配合研究利什曼原虫 (Leishmania)的金属抗药性 ,本文建立了一种氢化物发生 -原子荧光法测定细胞内痕量锑的方法。在选定最佳仪器条件下测定 ,锑的线性范围达到 0～ 2 0 μg L ,检出限为 0 0 8μg L ,加标回收率为 92 8% - 10 3 6 %。此方法操作简便、灵敏度高、费用低廉。所获结果与电感耦合等离子体质谱 (ICP MS)测试结果进行了比较 ,经配对t检验t=0 0 0 1<t0 0 5,10 =2 2 2 8(P >0 0 5 ) ,两者无显著性差异。该方法已应用于寄生虫的金属抗药性研究 ,获得满意的结果。An new and simple method to determine the antimony (Sb) in cells using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) was established for studying the mechanism of metal resistance in the protozoan parasite Leishmania. The detection limit was 0.08μg/L respectively. The average recoveries at different concentrations were 92.8%-103.6%. This method was simple, precise,reliable. The obtained results were compared with those determined by ICP-MS, and no obvious differences could be observed. This method has been successfully applied to the mechanism study of metal resistance in Leishmania.

关 键 词：氢化物发生-原子荧光 细胞 锑 利什曼原虫 

分 类 号：R382.22[医药卫生—医学寄生虫学] R965.2[医药卫生—基础医学]