不同组织来源透明质酸的分离纯化及收率比较  

The Purification and Comparison of Production-rate of Hyaluronic Acid Isolated from Various Tissues

作　　者：张谦朋[1] 赵子鹏 陈倩[1] 赵隽咏[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]冠县实验中学,山东冠县252500

出　　处：《聊城大学学报（自然科学版）》2004年第1期51-53,共3页Journal of Liaocheng University:Natural Science Edition

摘　　要：以鸡冠和人脐带为原料,分别利用水浸提乙醇沉淀法以及酶解法提取透明质酸粗品。前者经DEAE-纤维素纯化得高纯度的透明质酸,后者经乙酸钠纯化得纯品。经测定,红外扫描图谱与文献报道一致。A pure high molecular weight hyaluronic acid was isolated from rooster comb and humen umbilcal cord seperately. The former's process invelved extraction with water, precipitation by ethanol. purification with DEAE-cellulose; the later's extraction with enzyme, precipitation also by thtanol. purification with Sodium acetate. By comparation the productivity from rooster comb is higher than from human umbilical cord. Their infrared spectrum analysis was in accord with that reported in the literature.

关 键 词：透明质酸 DEAE-纤维素 脐带 鸡冠 氯代十六烷基毗啶 分离 纯化 多糖 

分 类 号：Q53[生物学—生物化学] Q599