川芎嗪对急性放射损伤小鼠骨髓碱性成纤维细胞生长因子及其受体表达水平的影响  

Effect of Ligustrazine on Expressions of Basic Fibroblast Growth Factor and Its Receptor in Bone Marrow of Mice with Acute Radiation Injury

作　　者：吴宁[1] 孙汉英[1] 刘文励[1] 孟凡凯[1] 刘振芳[1] 徐慧珍[1] 路武[1] 谢瑶[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030

出　　处：《中国中西医结合杂志》2004年第5期439-441,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.39870926)

摘　　要：目的 探讨急性放射损伤小鼠骨髓碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)及其受体(basic fibroblast growth factor receptor,bFGFR)的表达及川芎嗪对其影响。方法 56只清洁级昆明小鼠,随机分为3组:正常组,急性放射损伤对照组,川芎嗪组。将对照组和川芎嗪组小鼠用6.0Gy60Corγ射线一次性全身均匀照射,吸收剂量率为0.56Gy/min。照射后即分别胃饲生理盐水0.2ml/只及川芎嗪2mg/只,每天2次,连续13天。分别于照射后第3、7、14天颈椎脱臼法处死小鼠,取双侧股骨骨髓细胞制备单个核细胞(bone marrow mono-nuclear cells,BMMNC)悬液,用流式细胞仪检测小鼠BMMNC表面bFGFR的表达水平;取单侧肱骨切片后用免疫组织化学sABC-AP法检测小鼠骨髓组织中bFGF的表达水平。结果 急性放射损伤后第3、7、14天,对照组、川芎嗪组小鼠骨髓组织中bFGF表达显著低于正常组(P<0.05或P<0.01),同一时间点川芎嗪组bFGF、bFGFR的表达均显著高于对照组(P<0.05或P<0.01)。结论 川芎嗪通过促进骨髓组织中bFGF、BMMNC表面bFGFR的表达加速骨髓造血微环境的修复,可能是川芎嗪促进急性放射损伤后骨髓造血重建机制之一。To study the expressions of basic fibroblast growth factor (bFGF) and its receptor (bFGFR) in bone marrow of mice with acute radiation injury, and to evaluate the effect of Ligustrazine (Lt) on them. Methods Fifty-six Kunming mice of clean grade were randomly divided into 3 groups, the normal group, the control group and the Lt group. Mice in the latter two groups were once homogeneously systemic irradiated with 6.0 Gy of to, with the absorption dose rate of 0. 56 Gy/min, then treated with saline (0. 2 ml/mice) or Lt (2 mg/ mice) respectively, twice a day through gastrogavage for successive 13 days. Mice were sacrificed in batch on the 3rd, 7th and 14th day by cervical dislocation to collect the bilateral femoral bone marrow for preparing bone marrow mono-nuclear cell (BMMNC) suspension. The bFGFR expression on surface of BMMNC was determined by flow cytometry; and the bFGF expression level in one side of femoral bone marrow tissue was detected by immunohistochemistry with SABC-AP assay. Results The bFGF expression in bone marrow of mice on the 3rd, 7th and 14th day after acute radiation injury all were significantly lower than that of the normal mice (P< 0.05 or P<0.01). The expressions of bFGF and bFGFR in the Lt group detected were significantly higher than that in the control group detected at the corresponding time points (P < 0.05 or P < 0.01). Conclusion By way of enhancing bFGF expression in bone marrow and bFGFR expression on surface of BMMNC to accelerate the repairing of homopoietic micro-environment in bone marrow might be one of the mechanisms of Lt in promoting hemopoietic function reconstitution after acute radiation injury.

关 键 词：川芎嗪 急性放射损伤 小鼠 骨髓 碱性成纤维细胞生长因子受体 bFGF 受体表达 造血微环境 

分 类 号：R285[医药卫生—中药学]