工频电磁场对小鼠骨髓间充质干细胞BMP-2和TGF-β_1 mRNA表达的影响  

50Hz electromagnetic field (EMF) stimulation promotes mRNA expression of BMP-2 and TGF-β_1 in mouse bone marrow mesenchymal stem cells in vitro

作　　者：葛保健[1] 方真华[1] 赵文春[2] 任凯[1] 冷燕奎[1] 吴华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉430030 [2]海军工程大学电力电子技术应用研究所

出　　处：《中华物理医学与康复杂志》2004年第5期262-265,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金主任基金 (No.50 3470 2 5)

摘　　要：目的　研究工频电磁场对体外培养的小鼠骨髓间充质干细胞BMP 2和TGF β1mRNA表达的影响。方法　体外分离培养小鼠骨髓间充质干细胞 ,取第 2代细胞分组暴磁 ,然后用RT PCR技术分别检测各组细胞的BMP 2和TGF β1mRNA表达 ,并进行定量分析。结果　适当强度及作用时间的工频电磁场刺激可使小鼠骨髓间充质干细胞mRNA表达明显增强。相同作用时间下 ,BMP 2mRNA的表达于 1.6mT场强中达最大值 ,而TGF β1mRNA的表达则于 0 .4mT场强中达最大值 ;1.6mT场强条件下 ,电磁场连续照射 5d ,每日 1h ,可使BMP 2和TGF β1mRNA表达均达到最大值。结论　适当“窗口”的电磁场刺激对体外培养的小鼠骨髓间充质干细胞BMP 2和TGFObjective To study the effects of 50Hz electromagnetic field (EMF) on mRNA expression of BMP 2 and TGF β 1 in mouse bone marrow mesenchymal stem cells (MSCs) in vitro. Methods the mouse bone marrow mesenchymal stem cells were isolated and cultured in vitro. The second passage cells were harvested and divided into different groups. 50Hz EMF stimulation was conducted accordingly. The expression of mRNA of BMP 2 and TGF β 1 in the cells in every group was examined by reverse transcriptase polymerase chain reaction (RT PCR). Results Various intensities and times of exposure of 50Hz EMF induced a significant increase of BMP 2 and TGF β 1 mRNA in comparison to the controls. The expression of BMP 2 mRNA peaked with the stimulation intensity of 1.6mT . The corresponding intensity for TGF β 1 peak expression was 0.4mT. Both BMP 2 expression and TGF β 1 mRNA expression were increased to peak in cells when exposed continuously for 60 minutes per day for 5 days. Conclusion Stimulation of 50Hz EMF can increase significantly the expression of BMP 2 and TGF β 1mRNA in mouse bone marrow mesenchymal stem cells in vitro.

关 键 词：工频电磁场 小鼠 骨髓间充质干细胞 BMP-2 TGF-Β1 mRNA 逆转录PCR 

分 类 号：R454.1[医药卫生—治疗学]