146株念珠菌的检出分布与耐药分析  

Study on the Canidida spp infection and yeast sensitivity to antifungal agents by using E-test

作　　者：陈桂山[1] 梁锦胜[1] 卢兰芬[1] 吴秀娟[1] 吴剑扬[1] 

机构地区：[1]中山市人民医院检验中心,广东中山528403

出　　处：《检验医学》2004年第3期225-227,共3页Laboratory Medicine

摘　　要：目的　了解近期我院念珠菌感染和耐药情况。方法　收集并分析我院临床标本分离的念珠菌 14 6株 ,用E test对分离株进行 5种常用抗真菌药的体外敏感性检测。结果　临床念珠菌感染以呼吸道最为严重 ,占 4 7.9% ,其次为尿道 (2 3.9% )、肠道 (8.9% )、生殖道 (8.9% )。菌种分布 :白色念珠菌为 5 9.6 % ,热带念珠菌为 2 4 .6 % ,光滑念珠菌为 7.5 %。对 5种常用药物 (氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、酮康唑 )进行耐药性分析 ,发现氟胞嘧啶、两性霉素B对试验念珠菌具有良好的体外抑菌活性 ,其MIC50 、MIC90 值较低 ;唑类药物对试验念珠菌体外抑菌活性较差 ,其MIC50 、MIC90 值较高。结论　临床上念珠菌感染仍以白色念珠菌引起的呼吸道感染为主。用E test检测念珠菌对抗真菌药体外敏感性表明 ,唑类药物耐药现象比氟胞嘧啶、两性霉素严重。EObjective To study the Canidida spp infection and yeast resistant to antifungal agents in our hospital recently. Methods Collecting 146 isolates from clinical specimen and using E test to test antifungal susceptibility of Canidida spp. Results The prevalence rate of Canidida spp infection was: C albicans (59.6%); C tropicalis (24.6%); C glabrata (7.5%). Results of bacteriostasis test in vitro showed that 5FC, AMB were better. Their MIC 50 , MIC 90 were lower, and those of azone drug were higher. Conclusions The C albicans are the main infection strains, and show low resistant to 5FC, AMB; high resistant to azole drug. E test as an antifungal susceptibility test is conveniently performed in clinical laboratory.

关 键 词：念珠菌 耐药性 抗真菌药 浓度梯度法 

分 类 号：R379.4[医药卫生—病原生物学]