罗城汉族、壮族和仫佬族儿童HLA-DQA1多态性与幽门螺杆菌感染相关性的研究  

The association of HLA-DQA1 polymorphism with Helicobacter pylori infection in children of Guangxi Luocheng Han, Zhuang and Mulao nationalities

作　　者：王琳琳[1] 陈亭平[2] 林伟雄[1] 姜海行[1] 单庆文[1] 谢湘芝[1] 梁淡湄[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021 [2]广西医科大学

出　　处：《广西医学》2004年第6期778-781,共4页Guangxi Medical Journal

基　　金：广西自然科学基金资助项目 (批准文号 :桂科自 0 135 0 2 6 )

摘　　要：目的　了解广西罗城县汉族、壮族和仫佬族儿童的HLA DQA1等位基因多态性及其与幽门螺杆菌 (Helicobacterpylori,Hp)感染状况的关系。方法　 (1)对 36例汉族、5 4例壮族和 76例仫佬族儿童采用间接酶联免疫吸附法 (ELISA)测定抗Hp IgG抗体和免疫印迹法检测Hp空泡毒素 (CagA)、毒素相关蛋白 (VacA)及尿素酶抗体 ;(2 )采用聚合酶链反应 -序列特异引物 (PCR SSP)方法对三个民族儿童的HLA DQA1等位基因进行检测 ,并采用疾病关联分析法进行HLA DQA1等位基因分布与Hp感染检测结果相关性分析。 结果　 (1)罗城汉族儿童Hp阳性率为 77 8% ,壮族儿童为 6 4 8%、仫佬族儿童为 5 7 9% ,各民族之间Hp阳性率差异无显著性意义 ;(2 ) 3个人群中HLA DQA1等位基因分布比较接近 ,其中HLA DQA1 0 10 4等位基因检出频率最高。用疾病关联分析法进行HLA DQA1等位基因分布与Hp感染相关性分析 ,发现三个民族中Hp阳性儿童和Hp阴性儿童在HLA DQA1 0 10 4等位基因上存在差异 ,HLA DQA1 0 10 4等位基因可能是Hp感染的易感基因。 结论　罗城汉族、壮族、仫佬族儿童Hp感染率较高 ,但各民族间无显著差异 ;HLA DQA1 0 10 4等位基因可能是Hp感染的易感基因。Objective To analyse the association of HLA-DQA1 polymorphism of Guangxi Han、Zhuang and Mulao nationalities with Helicobacter pylori infection.Methods (1)Carried out serodiagnosis for determining Hp antibody by ELISA and CagA, VacA and Urease antibodies by immunobloting to diagnose H. pylori infection for 36 (Han), 54 (Zhuang) and 76 (Mulao) children. (2)Applied PCR-SSP technique to determine the polymorphism of the HLA-DQA1 locus of them and then analysed the association of HLA-DQA1 alleles of these nationalities children with Helicobacter pylori infection by SAS soft ware.Results The prevalence of Hp infection are 77.8% in Han nationality, 64.8% in Zhuang nationality, and 57.9% in Mulao nationality.The distribution of HLA-DQA1 loci is not significant difference in 3 studied groups. HLA-DQA1*0104 locus is most common examined particularly in children with Hp infection (+) in 3 studied groups so maybe that associates with Hp infection.Conclusion The results indicate that the prevalence of Hp infection in Guangxi Han, Zhuang, Mulao nationalities is high. HLA-DQA1*0104 locus is most common examined and which is considered the locus that associates with Hp infection.

关 键 词：人类白细胞抗原 HLA-DQA1 PCR-SSP 民族 幽门螺菌 罗城汉族 壮族 仫佬族 儿童 细菌感染 

分 类 号：R516[医药卫生—内科学]