MMP-9和TIMP-1在实验性肺气肿大鼠肺组织中的表达及其意义  

Expression of metalloproteinase-9 and its tissue inhibitor-1 in lung of experimenatial pulmonary emphysema in rats

作　　者：程青[1] 王秋月[1] 许惟元[1] 

机构地区：[1]中国医科大学第一附属医院呼吸疾病研究所,辽宁沈阳110001

出　　处：《中国现代医学杂志》2004年第10期86-88,共3页China Journal of Modern Medicine

摘　　要：目的　研究基质金属蛋白酶(MMP-9)及其组织抑制因子(TIMP-1)在弹性蛋白酶所致肺气肿大鼠肺组织中的表达。方法　雄性Wistar大鼠20只,随机分为二组正常对照组和肺气肿模型组,每组10只。模型组大鼠气管内滴入弹性蛋白酶复制肺气肿模型。25d后,观察各组大鼠肺组织的病理改变,免疫组化方法观察肺组织MMP-9和TIMP-1中的蛋白表达情况。结果　肺气肿模型组MMP-9和TIMP-1的表达与正常对照组相比明显增强(P<0.01),且MMP-9/TIMP-1比值失衡。结论　MMP-9/TIMP-1失衡在肺气肿形成中起重要作用。Objective:To study the expression of metalloproteinase-9 (MMP-9) and its tissue inhibitor-1 (TIMP-1) in the lung of elastase-induced emphysema in rats.Methods:Twenty Wistar rats were randomly divided into two groups, the normal group and the model group, ten rats in each group. Emphysema rat models were established by intratracheal instillation elastase. Twenty-five days later, the pathological changes were observed, the expression of MMP-9 and TIM-1 in the lung tissue was verified by immunohistochemical analysis.Results: The expression of MMP-9, and TIMP-1 in model group were significantly increased than those of normal group ( P <0.01) and the ratio of MMP-9/TIMP-1 was unbalanced. Conclusions: The imbalance of MMP-9/TIMP-1 may play an important role in emphysema.

关 键 词：实验性肺气肿 基质金属蛋白酶-9(MMP-9) 基质金属蛋白酶组织抑制因子-1(TIMP-1) 弹性蛋白酶 

分 类 号：R563.3[医药卫生—呼吸系统]