Pax-6在大鼠发育脑喙侧神经干细胞迁移流中的表达  

EXPRESSIONS OF PAX-6 IN ROSTRAL NSCs MIGRATORY STREAM OF DEVELOPING RAT BRAIN

作　　者：邱克军[1] 蔡文琴[1] 姚忠祥[1] 杨辉[2] 周德山[1] 何家全[2] 刘仕勇[2] 

机构地区：[1]第三军医大学组织学胚胎学教研室,重庆400038 [2]第三军医大学附属新桥医院神经外科,重庆400037

出　　处：《解剖学报》2004年第3期262-267,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助重点研究项目 ( 3 0 13 0 110 )

摘　　要：目的　了解大鼠脑发育过程中 ,喙侧神经干细胞迁移流中不同时期Pax 6表达水平的时空差异。　方法　用BrdU对胚胎 14d、出生后 0、1和 7d的Wistar大鼠行活体标记 ,取各时相点鼠脑 ,冰冻切片 ,行BrdU、Nestin、Pax 6和胶质纤维酸性蛋白 (GFAP)免疫荧光染色 ,观察Pax 6在脑内增殖细胞中的表达情况 ,比较在上述 4个时相点 ,Pax 6于室管膜前下区、迁移流主干部分和嗅球 3个部分的表达差异。　结果　 1 在发育中大鼠脑内 ,Pax 6主要表达于神经干细胞内 ,在海马增殖细胞亦有较高表达。 2 在喙侧神经干细胞迁移流内 ,Pax 6在迁移流主干部分的表达高于室管膜前下区和嗅球 ;在胚胎 14d的表达与出生后 0d和 1d相似 ,表达水平较高 ,出生后 7d迅速下降到很低水平。　结论　在大鼠脑发育过程中 ,Pax 6主要表达于神经干细胞及海马增殖性细胞内 ,在大鼠出生后 ,随着大鼠脑的发育 ,Pax 6于喙侧神经干细胞迁移流的表达迅速减弱 ,但迁移流主干部分的表达始终高于室管膜前下区和嗅球。Objective To study the expressions of Pax-6 in rostral neural stem cells(NSCs)migratory stream at different time courses of developing rat brains. Methods Embryonic day 14(E14),postnatal day 0(P0),postnatal day 1(P1)and postnatal day 7(P7)Wistar rats were labeled with BrdU in vivo.All rat brains were collected and cryostated,followed by immunofluorescence stained with BrdU,nestin,Pax-6 and GFAP antibodies.The expressions of Pax-6 in BrdU labeled cells were studied.The expressions of Pax-6 were compared in anterior subventricular zone(SVZa),rostral migratory stream(RMS) and olfactory bulb(OB) in E14,P0,P1 and P7 brains of Wistar rats. Results 1.The expressions of Pax-6 were mainly localized in NSCs and hippocampus proliferating cells of developing rat brains;2.The expressions of Pax-6 were higher in RMS than that of SVZa or OB in E14,P0,P1 and P7 rat brains;3.The expressions of Pax-6 were similar in E14,P0 and P1 rat brains,and higher than that of P7.Conclusions Pax-6 expresses mainly localized in NSCs and hippocampus proliferating cells of developing rat brain. The expressions of Pax-6 reduce rapidly in postnatal rat brain.The expressions of Pax-6 in RMS are always higher than that of SVZa and OB regions.The significance of the different expression patterns is dicussed.;

关 键 词：神经干细胞 喙侧迁移流 PAX-6 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]