人肿瘤坏死因子α基因对绒毛膜癌耐药细胞凋亡影响的体内研究  

IN VIVO STUDY OF THE APOPTOSIS IN TRANSFECTION OF HUMAN TUMOR NECROSIS FACTOR-α INTO DRUG RESISTANT CELL LINE OF CHORIOCARCINOMA

作　　者：张卫光[1] 冯凤芝[2] 向阳[2] 肖军军[3] 夏家骝[1] 

机构地区：[1]北京大学医学部解剖学与组织学胚胎学系,北京100083 [2]中国医学科学院中国协和医科大学北京协和医院妇产科,北京100730 [3]北京大学医学部细胞生物学系,北京100083

出　　处：《解剖学报》2004年第3期268-271,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 0 0 0 179) ;北京大学"985"跨学科资助项目

摘　　要：目的　研究转染人肿瘤坏死因子α(huTNF α)基因对裸鼠绒毛膜癌 (绒癌 )耐药细胞移植瘤组织细胞凋亡的影响。　方法　用人绒癌耐药细胞系JEG 3 VP2和转染了huTNF α的绒癌耐药细胞系JEG 3 VP2 huTNF接种裸鼠各 6只 ,行足叶乙甙 (VP16 )化疗后 ,采用RT RCR、HE染色和TUNEL检测方法 ,观察移植瘤细胞的凋亡情况。结果　在接种JEG 3 VP2 huTNF α的移植瘤组织中 ,存在huTNF α基因的表达 ;JEG 3 VP2 huTNF α组出现凋亡小体的细胞和TUNEL阳性细胞明显多于JEG 3 VP2组 ,P <0 0 5。　结论　转染huTNF α基因促进了绒癌耐药细胞的凋亡。Objective To investigate the apoptosis of a choriocarcinoma drug-resistant cell line transfected with human tumor necrosis factor-α(huTNF-α)gene in nude mice model. Methods Six nude mice each group were injected subcutaneously with drug-resistant choriocarcinoma cell line JEG-3/VP2,and huTNF-α-transfected cell line(JEG-3/VP2/huTNF-α),respectively.After VP16 chemotherapy,reverse transcription-polymerase chain reaction(RT-PCR),hematoxylin-eosine(HE)stain and TUNEL method were applied to study the apoptosis in choriocarcinoma implant tumor cells. Results Expression of huTNF-α gene was observed in implant tumor tissue with JEG-3/VP2/huTNF-α cell line.The number of the cells with apoptosis body and the TUNEL(+) cells in JEG-3/VP2/huTNF-α group were significantly more than those in JEG-3/VP2 group.Conclusion The huTNF-α gene can promote the apoptosis of drug-resistant choriocarcinoma of JEG-3/VP2 cell line.;

关 键 词：肿瘤坏死因子 绒毛膜癌 细胞凋亡 裸鼠 

分 类 号：R711[医药卫生—妇产科学]