大鼠肝硬化门腔静脉分流术后运动皮层和脊髓一氧化氮合酶阳性神经元的变化  

CHANGES OF NOS POSITIVE NEURONS IN CORTEX MOTOR AREA AND SPINAL CORD IN RATS OF CIRRHOSIS WITH PORTACAVAL SHUNTS

作　　者：万华瑛[1] 高秀来[1] 刘霞[1] 周馨[1] 

机构地区：[1]首都医科大学解剖学教研室,北京100054

出　　处：《解剖学报》2004年第3期320-323,共4页Acta Anatomica Sinica

摘　　要：目的　研究大鼠肝硬化门腔静脉分流术后运动皮层和脊髓一氧化氮合酶 (NOS)阳性神经元的变化。方法　采用NADPH d黄递酶组织化学法以及NOS荧光免疫组织化学技术结合激光共聚焦扫描显微镜方法。结果肝硬化门腔静脉分流术后运动皮层NOS和nNOS阳性细胞显著减少 ;脊髓NOS阳性细胞改变不明显。　结论　大鼠肝硬化门腔静脉分流可引起大脑皮层细胞的改变 ,NO可能参与了肝硬化门腔静脉分流术后对中枢神经系统的损害。Objective To study the changes of NOS positive neurons in cortex motor area and spinal cord in rats of cirrhosis with portacaval shunts. Methods NADPH-diaphorase, fluorescence immunohistochemistry with confocal laser scanning microscope (CLSM) were used. Results The number of NOS and nNOS positive neurons in cirrhosis rats with portacaval shunts decreased obviously.Conclusion Cirrhosis with portacaval shunts can influence motor neurons of cortex; NO may participate in the central nervous system changes of cirrhosis rats with portacaval shunts.;

关 键 词：肝硬化门腔静脉分流 大脑皮层运动细胞 一氧化氮合酶 激光共聚焦扫描显微镜 大鼠 

分 类 号：R3[医药卫生—基础医学]