蛇毒蛋白C激活剂实验条件及稳定性的探讨  

Research on the Experimental Conditions and Stability of Protein C Activator Isolated from Snake Venom

作　　者：侯力强[1] 赵路宁[1] 黄韶玲[1] 何泉华[1] 江中喜[2] 孔天翰[1] 管锦霞[1] 

机构地区：[1]广州医学院蛇毒研究所,广东广州510182 [2]广州医学院第二附属医院,广东广州510260

出　　处：《现代检验医学杂志》2004年第3期12-14,共3页Journal of Modern Laboratory Medicine

基　　金：广州市教委科研项目基金;广东省医学科学技术研究基金资助项目 ( B2 0 0 0 0 61)

摘　　要：目的　探讨可能影响蛇毒蛋白 C激活剂作用的实验条件和该试剂的稳定性。方法　分别采用APTT法和 CBS0 2 .46底物法 ,检测蛇毒蛋白 C激活剂在不同实验条件下的抗凝活性及酰胺酶活性。结果　蛇毒蛋白 C激活剂的最适 p H值为 6.0～ 7.0 ,最适温度为 37～ 40℃ ,肝素、枸橼酸钠等抗凝剂及 Na+ 、K+ 、EDTA等不影响其活性 ,SDS则显著抑制其生物学活性。该试剂溶液剂型在室温下或 4℃中 4w内效价无变化 ,冻干剂型则在 2 y内效价无明显变化。结论　蛇毒蛋白 C激活剂是一种效价较高、稳定性较好、影响因素少的蛋白Objective To investigate the experimental conditions and stability that may affect the activities of Protein C activator(PCA-SV).Methods PCA-SV's possible anticoagulant and amidase activities were measured by method of APTT and that of specific chromogenic substrate respectively in various experimental conditions.Results The pH-optimum and temperature-optimum for the action of PCA-SV were 6.0～7.0 and 37～40 ℃ respectively.The anticoagulants(heparin and Sodium Citrate) had no effect on the activities.SDS significantly inhibited the biological action of PCA-SV.In room temperature or 4 ℃,the titre had no change within 4 weeks for solution reagent and 2 years for dry reagent.Conclusion PCA-SV is a new protein C-activating reagent that has high potency and stability.

关 键 词：蛇毒 蛋白C 激活剂 活化蛋白C抵抗症 

分 类 号：R446[医药卫生—诊断学]